ArticleClinical epigenetics2016
Next-generation sequencing methylation profiling of subjects with obesity identifies novel gene changes.
Article in Clinical epigenetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Molecular Effects of Physical Activity and Body Composition: A Systematic Review and Meta-Analysis.International journal of environmental research and public health · 2025Pooled it
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- Homocysteine interferes with Ndufa1 leading to mitochondrial dysfunction through repression of the NADCell death & disease · 2025Article
- Genome-Wide Association Study of Gallstone Disease Identifies Novel Candidate Genomic Variants in a Latino Community of Southwest USA.Journal of racial and ethnic health disparities · 2025Article
- Exon 1 methylation status of CDH13 is associated with decreased overall survival and distant metastasis in patients with postoperative colorectal cancer.Discover oncology · 2024Article
- Can Exercise Training Alter Human Skeletal Muscle DNA Methylation?Metabolites · 2022Article
- Weight loss after Roux-En-Y gastric bypass surgery reveals skeletal muscle DNA methylation changes.Clinical epigenetics · 2021Article
- Enhanced epigenetic profiling of classical human monocytes reveals a specific signature of healthy aging in the DNA methylome.Nature aging · 2021Article
- Genome-wide profiling of DNA methylation and gene expression identifies candidate genes for human diabetic neuropathy.Clinical epigenetics · 2020Article
- Dynamic changes of muscle insulin sensitivity after metabolic surgery.Nature communications · 2019Article
- A coding and non-coding transcriptomic perspective on the genomics of human metabolic disease.Nucleic acids research · 2018Article
- Epigenomics and metabolomics reveal the mechanism of the APOA2-saturated fat intake interaction affecting obesity.The American journal of clinical nutrition · 2018Article
- Role of DNA methylation in altered gene expression patterns in adult zebrafish (Environmental epigenetics · 2018Article
- Potential epigenetic biomarkers of obesity-related insulin resistance in human whole-blood.Epigenetics · 2017Article
- Alterations of sorbin and SH3 domain containing 3 (SORBS3) in human skeletal muscle following Roux-en-Y gastric bypass surgery.Clinical epigenetics · 2017Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundObesity is a metabolic disease caused by environmental and genetic factors. However, the epigenetic mechanisms of obesity are incompletely understood. The aim of our study was to investigate the role of skeletal muscle DNA methylation in combination with transcriptomic changes in obesity.
resultsMuscle biopsies were obtained basally from lean (n = 12; BMI = 23.4 ± 0.7 kg/m(2)) and obese (n = 10; BMI = 32.9 ± 0.7 kg/m(2)) participants in combination with euglycemic-hyperinsulinemic clamps to assess insulin sensitivity. We performed reduced representation bisulfite sequencing (RRBS) next-generation methylation and microarray analyses on DNA and RNA isolated from vastus lateralis muscle biopsies. There were 13,130 differentially methylated cytosines (DMC; uncorrected P < 0.05) that were altered in the promoter and untranslated (5' and 3'UTR) regions in the obese versus lean analysis. Microarray analysis revealed 99 probes that were significantly (corrected P < 0.05) altered. Of these, 12 genes (encompassing 22 methylation sites) demonstrated a negative relationship between gene expression and DNA methylation. Specifically, sorbin and SH3 domain containing 3 (SORBS3) which codes for the adapter protein vinexin was significantly decreased in gene expression (fold change -1.9) and had nine DMCs that were significantly increased in methylation in obesity (methylation differences ranged from 5.0 to 24.4 %). Moreover, differentially methylated region (DMR) analysis identified a region in the 5'UTR (Chr.8:22,423,530-22,423,569) of SORBS3 that was increased in methylation by 11.2 % in the obese group. The negative relationship observed between DNA methylation and gene expression for SORBS3 was validated by a site-specific sequencing approach, pyrosequencing, and qRT-PCR. Additionally, we performed transcription factor binding analysis and identified a number of transcription factors whose binding to the differentially methylated sites or region may contribute to obesity.
conclusionsThese results demonstrate that obesity alters the epigenome through DNA methylation and highlights novel transcriptomic changes in SORBS3 in skeletal muscle.
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