Evidence map›Paper›PMID 27436904›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Probing the structure of Nun transcription arrest factor bound to RNA polymerase.

Arkady Mustaev, Christal L Vitiello, Max E Gottesman

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Transcription elongation.Transcription · 2017
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Arkady MustaevPublic Health Research Institute, Newark, NJ 07103; Department of Microbiology and Molecular Genetics, Rutgers New Jersey Medical School, Rutgers Biomedical and Health Sciences, Newark, NJ 07103;
Christal L VitielloDepartment of Microbiology & Immunology, Columbia University Medical Center, New York, NY 10032.
Max E GottesmanDepartment of Microbiology & Immunology, Columbia University Medical Center, New York, NY 10032 meg8@columbia.edu.
Columbia University Irving Medical Center · USRutgers Health

Funding

CONTROL OF TRANSCRIPTION TERMINATION IN E. COLIR01GM037219 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI GOTTESMAN, MAX ELLIOT · 1986 to 2017
$6.6M
NIGMS NIH HHS R01 GM037219
6 · The paper itself

Abstract

The coliphage HK022 protein Nun transcription elongation arrest factor inhibits RNA polymerase translocation. In vivo, Nun acts specifically to block transcription of the coliphage λ chromosome. Using in vitro assays, we demonstrate that Nun cross-links RNA in an RNA:DNA hybrid within a ternary elongation complex (TEC). Both the 5' and the 3' ends of the RNA cross-link Nun, implying that Nun contacts RNA polymerase both at the upstream edge of the RNA:DNA hybrid and in the vicinity of the catalytic center. This finding suggests that Nun may inhibit translocation by more than one mechanism. Transcription elongation factor GreA efficiently blocked Nun cross-linking to the 3' end of the transcript, whereas the highly homologous GreB factor did not. Surprisingly, both factors strongly suppressed Nun cross-linking to the 5' end of the RNA, suggesting that GreA and GreB can enter the RNA exit channel as well as the secondary channel, where they are known to bind. These findings extend the known action mechanism for these ubiquitous cellular factors.

Indexed as

Transcription, GeneticAmino Acid SequenceBacteriophage HK022DNA-Directed RNA PolymerasesGene Expression Regulation, ViralModels, MolecularNucleic Acid ConformationProtein BindingProtein DomainsRNA, ViralTranscriptional Elongation FactorsTranscription FactorsViral ProteinsDNA-Directed RNA PolymerasesNun protein, Enterobacteria phage HK022RNA, ViralTranscriptional Elongation FactorsTranscription FactorsViral ProteinsGre factorsNun proteinphage HK022ternary elongation complextranscription arrest

Identifiers

PMID27436904
PMCPMC4978253
OpenAlexW2483447806

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.