ArticleNucleic acids research2016
Length heterogeneity at conserved sequence block 2 in human mitochondrial DNA acts as a rheostat for RNA polymerase POLRMT activity.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Mechanisms and regulation of human mitochondrial transcription.Nature reviews. Molecular cell biology · 2024Review
- Article
- Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence.Vaccines · 2023Article
- TEFM variants impair mitochondrial transcription causing childhood-onset neurological disease.Nature communications · 2023Article
- Nuclear genetic control of mtDNA copy number and heteroplasmy in humans.medRxiv : the preprint server for health sciences · 2023Article
- Mitochondrial DNAFrontiers in ophthalmology · 2023Article
- Mitochondrial DNA variation across 56,434 individuals in gnomAD.Genome research · 2022Article
- Spectroscopic Characterization of Mitochondrial G-Quadruplexes.International journal of molecular sciences · 2022Article
- To Be a Champion of the 24-h Ultramarathon Race. If Not the Heart ... Mosaic Theory?International journal of environmental research and public health · 2021Article
- High expression of theJournal of gastrointestinal oncology · 2020Article
- MtDNA sequence features associated with 'selfish genomes' predict tissue-specific segregation and reversion.Nucleic acids research · 2020Article
- Mitochondrial Genetic Drift after Nuclear Transfer in Oocytes.International journal of molecular sciences · 2020Review
- Mitochondrial GWAS and association of nuclear - mitochondrial epistasis with BMI in T1DM patients.BMC medical genomics · 2020Article
- Evaluation of non-coding region sequence variants and mitochondrial haplogroups as potential biomarkers of sporadic breast cancer in individuals of Sri Lankan Sinhalese ethnicity.Biomedical reports · 2020Article
- Article
- DNA specificities modulate the binding of human transcription factor A to mitochondrial DNA control region.Nucleic acids research · 2019Article
- Structural basis of mitochondrial transcription.Nature structural & molecular biology · 2018Review
- Article
- Mitochondrial DNA replication: a PrimPol perspective.Biochemical Society transactions · 2017Review
- The human mitochondrial transcription factor A is a versatile G-quadruplex binding protein.Scientific reports · 2017Article
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4 authors.
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Abstract
The guanine (G)-tract of conserved sequence block 2 (CSB 2) in human mitochondrial DNA can result in transcription termination due to formation of a hybrid G-quadruplex between the nascent RNA and the nontemplate DNA strand. This structure can then influence genome replication, stability and localization. Here we surveyed the frequency of variation in sequence identity and length at CSB 2 amongst human mitochondrial genomes and used in vitro transcription to assess the effects of this length heterogeneity on the activity of the mitochondrial RNA polymerase, POLRMT. In general, increased G-tract length correlated with increased termination levels. However, variation in the population favoured CSB 2 sequences which produced efficient termination while particularly weak or strong signals were avoided. For all variants examined, the 3' end of the transcripts mapped to the same downstream sequences and were prevented from terminating by addition of the transcription factor TEFM. We propose that CSB 2 length heterogeneity allows variation in the efficiency of transcription termination without affecting the position of the products or the capacity for regulation by TEFM.
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