ArticleSmall (Weinheim an der Bergstrasse, Germany)2016
Fingerprinting of Peptides with a Large Channel of Bacteriophage Phi29 DNA Packaging Motor.
Article in Small (Weinheim an der Bergstrasse, Germany), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Decoding information stored in DNAs through single-molecule electrical detection platforms.Innovation (Cambridge (Mass.)) · 2025Review
- DNA/RNA hybrids avoid channel gating that leads to the continued packaging of numerous hybrids into the phi29 protein shell.Nucleic acids research · 2025Article
- Portable nanopore-sequencing technology: Trends in development and applications.Frontiers in microbiology · 2023Review
- Voltage controlled shutter regulates channel size and motion direction of protein aperture as durable nano-electric rectifier-----An opinion in biomimetic nanoaperture.Biomaterials · 2022Article
- Lysenin Channels as Sensors for Ions and Molecules.Sensors (Basel, Switzerland) · 2020Review
- Sensing with Nanopores and Aptamers: A Way Forward.Sensors (Basel, Switzerland) · 2020Review
- Label-Free Detection of Post-translational Modifications with a Nanopore.Nano letters · 2019Article
- Channel from bacterial virus T7 DNA packaging motor for the differentiation of peptides composed of a mixture of acidic and basic amino acids.Biomaterials · 2019Article
- Nano-channel of viral DNA packaging motor as single pore to differentiate peptides with single amino acid difference.Biomaterials · 2018Article
- Paving the way to single-molecule protein sequencing.Nature nanotechnology · 2018Review
- The Utility of Nanopore Technology for Protein and Peptide Sensing.Proteomics · 2018Review
- Engineering of protein nanopores for sequencing, chemical or protein sensing and disease diagnosis.Current opinion in biotechnology · 2018Review
- Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore.Nature communications · 2018Article
- Porphyrin-Assisted Docking of a Thermophage Portal Protein into Lipid Bilayers: Nanopore Engineering and Characterization.ACS nano · 2017Article
- Stochastic sensing of Angiotensin II with lysenin channels.Scientific reports · 2017Article
- Channel of viral DNA packaging motor for real time kinetic analysis of peptide oxidation states.Biomaterials · 2017Article
- Nanopore Sensing.Analytical chemistry · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Nanopore technology has become a highly sensitive and powerful tool for single molecule sensing of chemicals and biopolymers. Protein pores have the advantages of size amenability, channel homogeneity, and fabrication reproducibility. But most well-studied protein pores for sensing are too small for passage of peptide analytes that are typically a few nanometers in dimension. The funnel-shaped channel of bacteriophage phi29 DNA packaging motor has previously been inserted into a lipid membrane to serve as a larger pore with a narrowest N-terminal constriction of 3.6 nm and a wider C-terminal end of 6 nm. Here, the utility of phi29 motor channel for fingerprinting of various peptides using single molecule electrophysiological assays is reported. The translocation of peptides is proved unequivocally by single molecule fluorescence imaging. Current blockage percentage and distinctive current signatures are used to distinguish peptides with high confidence. Each peptide generated one or two distinct current blockage peaks, serving as typical fingerprint for each peptide. The oligomeric states of peptides can also be studied in real time at single molecule level. The results demonstrate the potential for further development of phi29 motor channel for detection of disease-associated peptide biomarkers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.