ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2016
The chlorophyll-deficient golden leaf mutation in cucumber is due to a single nucleotide substitution in CsChlI for magnesium chelatase I subunit.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 95 citations in OpenAlex.
- Map-based cloning and functional validation of ClChlH governing yellow rind color in watermelon (Citrullus lanatus).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Map-based cloning identifies a missense SNP in CsTRX z, encoding a z-type thioredoxin homolog, as the genetic determinant of Dominant Virescent Leaf in cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Deletion of the gene encoding the magnesium chelatase I subunit resulted in a novel wheat leaf colour mutant.Plant biotechnology journal · 2025Article
- A Comprehensive Review: Genetic Mapping of Genes Associated with Green Leaf Color Variations in Main Vegetable Crops.Plants (Basel, Switzerland) · 2025Review
- Regreening mechanisms in cucumber: insights from a CsSIG2 mutation affecting chloroplast development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Transcriptome Analysis Reveals the Molecular Mechanism of the Leaf Yellowing in Allotriploid Cucumber.Genes · 2024Article
- Molecular Mechanisms of Chlorophyll Deficiency inPlants (Basel, Switzerland) · 2024Article
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- Photosynthetic characteristics and genetic mapping of a new yellow leaf mutantMolecular breeding : new strategies in plant improvement · 2023Article
- Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon.Scientific reports · 2023Article
- Melon yellow-green plant (Cmygp) encodes a Golden2-like transcription factor regulating chlorophyll synthesis and chloroplast development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Genetic mapping and molecular characterization of the delayed green geneFrontiers in plant science · 2023Article
- Fine mapping of CscpFtsY, a gene conferring the yellow leaf phenotype in cucumber (Cucumis sativus L.).BMC plant biology · 2022Article
- Genetic mapping and physiological analysis of chlorophyll-deficient mutant in Brassica napus L.BMC plant biology · 2022Article
- Identification and characterization ofHorticulture research · 2022Article
- Mapping and functional verification of leaf yellowing genes in watermelon during whole growth period.Frontiers in plant science · 2022Article
- Integrating physiology, genetics, and transcriptome to decipher a new thermo-sensitive and light-sensitive virescent leaf gene mutant in cucumber.Frontiers in plant science · 2022Article
- Molecular Characterization of Mg-Chelatase CHLI Subunit in Pea (Frontiers in plant science · 2022Article
- QTL Mapping of Heat Tolerance in Cucumber (Plants (Basel, Switzerland) · 2021Article
- The Intron Retention VariantFrontiers in plant science · 2021Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
key messageThe cucumber chlorophyll-deficient golden leaf mutation is due to a single nucleotide substitution in the CsChlI gene for magnesium chelatase I subunit which plays important roles in the chlorophyll biosynthesis pathway. The Mg-chelatase catalyzes the insertion of Mg(2+) into the protoporphyrin IX in the chlorophyll biosynthesis pathway, which is a protein complex encompassing three subunits CHLI, CHLD, and CHLH. Chlorophyll-deficient mutations in genes encoding the three subunits have played important roles in understanding the structure, function and regulation of this important enzyme. In an EMS mutagenesis population, we identified a chlorophyll-deficient mutant C528 with golden leaf color throughout its development which was viable and able to set fruits and seeds. Segregation analysis in multiple populations indicated that this leaf color mutation was recessively inherited and the green color showed complete dominance over golden color. Map-based cloning identified CsChlI as the candidate gene for this mutation which encoded the CHLI subunit of cucumber Mg-chelatase. The 1757-bp CsChlI gene had three exons and a single nucleotide change (G to A) in its third exon resulted in an amino acid substitution (G269R) and the golden leaf color in C528. This mutation occurred in the highly conserved nucleotide-binding domain of the CHLI protein in which chlorophyll-deficient mutations have been frequently identified. The mutant phenotype, CsChlI expression pattern and the mutated residue in the CHLI protein suggested the mutant allele in C528 is unique among mutations identified so far in different species. This golden leaf mutant not only has its potential in cucumber breeding, but also provides a useful tool in understanding the CHLI function and its regulation in the chlorophyll biosynthesis pathway as well as chloroplast development.
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