Evidence map›Paper›PMID 27435585›Full record

ArticleMolecular & cellular proteomics : MCP2016

Identification of Oligosaccharides in Feces of Breast-fed Infants and Their Correlation with the Gut Microbial Community.

Jasmine C C Davis, Sarah M Totten, Julie O Huang, Sadaf Nagshbandi, Nina Kirmiz, Daniel A Garrido, Zachery T Lewis, Lauren D Wu, Jennifer T Smilowitz, J Bruce German and 2 more

Registry-linked trialOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01817127 (Functional Deconstruction of Human Milk Oligosaccharides and Lipids), which is not on this map. Cited by 55 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
55citing papers in PubMed, 2 pooled it
7.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01817127 active not recruitingnot on this map

Functional Deconstruction of Human Milk Oligosaccharides and Lipids

TypeobservationalSponsorUniversity of California, DavisRan2009 to 2028Enrolled395ConditionsHealthy, Gestational Diabetes Mellitus, Type 2 Diabetes Mellitus
3 · Its place in the literature

Who cites it

55 citing papers in PubMed, 2 syntheses or guidelines pooled it, 102 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Jasmine C C DavisFrom the ‡Department of Chemistry, University of California, Davis, California 95616; §Foods for Health Institute, University of California, Davis, California 95616;
Sarah M TottenFrom the ‡Department of Chemistry, University of California, Davis, California 95616; §Foods for Health Institute, University of California, Davis, California 95616;
Julie O HuangFrom the ‡Department of Chemistry, University of California, Davis, California 95616;
Sadaf NagshbandiFrom the ‡Department of Chemistry, University of California, Davis, California 95616;
Nina Kirmiz§Foods for Health Institute, University of California, Davis, California 95616; ¶Department of Food Science and Technology, University of California, Davis, California 95616;
Daniel A Garrido§Foods for Health Institute, University of California, Davis, California 95616; ‖Department of Viticulture and Enology, University of California, Davis, California 95616.
Zachery T Lewis§Foods for Health Institute, University of California, Davis, California 95616; ¶Department of Food Science and Technology, University of California, Davis, California 95616;
Lauren D WuFrom the ‡Department of Chemistry, University of California, Davis, California 95616; §Foods for Health Institute, University of California, Davis, California 95616;
Jennifer T Smilowitz§Foods for Health Institute, University of California, Davis, California 95616; ¶Department of Food Science and Technology, University of California, Davis, California 95616;
J Bruce German§Foods for Health Institute, University of California, Davis, California 95616; ¶Department of Food Science and Technology, University of California, Davis, California 95616;
David A Mills§Foods for Health Institute, University of California, Davis, California 95616; ¶Department of Food Science and Technology, University of California, Davis, California 95616; ‖Department of Viticulture and Enology, University of California, Davis, California 95616.
Carlito B LebrillaFrom the ‡Department of Chemistry, University of California, Davis, California 95616; §Foods for Health Institute, University of California, Davis, California 95616; cblebrilla@ucdavis.edu.
University of California, Davis · US

Funding

Activation of probiotic bifidobacteria by milk glyansR01AT008759 · NCCIH · UNIVERSITY OF CALIFORNIA AT DAVIS · PI GERMAN, BRUCE, LEBRILLA, CARLITO B · 2014 to 2018
$4.2M
Mechanism of Activation of Probiotic Bifidobacteria by Prebiotic Milk GlycansR01AT007079 · NCCIH · UNIVERSITY OF CALIFORNIA AT DAVIS · PI GERMAN, BRUCE, LEBRILLA, CARLITO B · 2012 to 2016
$2.2M
Structures, Functions, & Genetic Diversity of Oligosaccharides in MilkR01HD061923 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEBRILLA, CARLITO B · 2009 to 2013
$1.6M
NCCIH NIH HHS R01 AT007079NCCIH NIH HHS R01 AT008759NICHD NIH HHS R01 HD061923
6 · The paper itself

Abstract

Glycans in breast milk are abundant and found as either free oligosaccharides or conjugated to proteins and lipids. Free human milk oligosaccharides (HMOs) function as prebiotics by stimulating the growth of beneficial bacteria while preventing the binding of harmful bacteria to intestinal epithelial cells. Bacteria have adapted to the glycan-rich environment of the gut by developing enzymes that catabolize glycans. The decrease in HMOs and the increase in glycan digestion products give indications of the active enzymes in the microbial population. In this study, we quantitated the disappearance of intact HMOs and characterized the glycan digestion products in the gut that are produced by the action of microbial enzymes on HMOs and glycoconjugates from breast milk. Oligosaccharides from fecal samples of exclusively breast-fed infants were extracted and profiled using nanoLC-MS. Intact HMOs were found in the fecal samples, additionally, other oligosaccharides were found corresponding to degraded HMOs and non-HMO based compounds. The latter compounds were fragments of N-glycans released through the cleavage of the linkage to the asparagine residue and through cleavage of the chitobiose core of the N-glycan. Marker gene sequencing of the fecal samples revealed bifidobacteria as the dominant inhabitants of the infant gastrointestinal tracts. A glycosidase from Bifidobacterium longum subsp. longum was then expressed to digest HMOs in vitro, which showed that the digested oligosaccharides in feces corresponded to the action of glycosidases on HMOs. Similar expression of endoglycosidases also showed that N-glycans were released by bacterial enzymes. Although bifidobacteria may dominate the gut, it is possible that specific minority species are also responsible for the major products observed in feces. Nonetheless, the enzymatic activity correlated well with the known glycosidases in the respective bacteria, suggesting a direct relationship between microbial abundances and catabolic activity.

Indexed as

Bacterial ProteinsBifidobacteriumChromatography, LiquidFecesGastrointestinal MicrobiomeGlycoside HydrolasesHumansInfantMass SpectrometryMilk, HumanOligosaccharidesBacterial ProteinsGlycoside HydrolasesOligosaccharides

Identifiers

PMID27435585
PMCPMC5013312
OpenAlexW2478228532

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.