ArticleProceedings of the National Academy of Sciences of the United States of America2016
Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 102 citations in OpenAlex.
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- Teaming up to overcome challenges toward translation of new therapeutics for osteoarthritis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024Article
- Recent advancements in cartilage tissue engineering innovation and translation.Nature reviews. Rheumatology · 2024Review
- Hydrogel Encapsulation of Genome-Engineered Stem Cells for Long-Term Self-Regulating Anti-Cytokine Therapy.Gels (Basel, Switzerland) · 2023Article
- CircNFIX regulates chondrogenesis and cartilage homeostasis by targeting the miR758-3p/KDM6A axis.Cell proliferation · 2022Article
- Early Transcriptional Changes of Adipose-Derived Stem Cells (ADSCs) in Cell Culture.Medicina (Kaunas, Lithuania) · 2022Article
- Functional tissue engineering of articular cartilage for biological joint resurfacing-The 2021 Elizabeth Winston Lanier Kappa Delta Award.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Creating a Functional Biomimetic Cartilage Implant Using Hydrogels Based on Methacrylated Chondroitin Sulfate and Hyaluronic Acid.Gels (Basel, Switzerland) · 2022Article
- Cartilage tissue engineering: From proinflammatory and anti‑inflammatory cytokines to osteoarthritis treatments (Review).Molecular medicine reports · 2022Review
- Cryogel Scaffold-Mediated Delivery of Adipose-Derived Stem Cells Promotes Healing in Murine Model of Atrophic Non-Union.Frontiers in bioengineering and biotechnology · 2022Article
- Information and Scientific Impact of Advanced Therapies in the Age of Mass Media: Altmetrics-Based Analysis of Tissue Engineering.Journal of medical Internet research · 2021Article
- Transient receptor potential vanilloid 4 as a regulator of induced pluripotent stem cell chondrogenesis.Stem cells (Dayton, Ohio) · 2021Article
- Biological resurfacing in a canine model of hip osteoarthritis.Science advances · 2021Article
- A genome-engineered bioartificial implant for autoregulated anticytokine drug delivery.Science advances · 2021Article
- Optimized Media Volumes Enable Homogeneous Growth of Mesenchymal Stem Cell-Based Engineered Cartilage Constructs.Tissue engineering. Part A · 2021Article
- A synthetic mechanogenetic gene circuit for autonomous drug delivery in engineered tissues.Science advances · 2021Article
- Recent advances in polymeric biomaterials-based gene delivery for cartilage repair.Bioactive materials · 2020Article
- Melt electrowriting onto anatomically relevant biodegradable substrates: Resurfacing a diarthrodial joint.Materials & design · 2020Article
- Scaffold-Mediated Gene Delivery for Osteochondral Repair.Pharmaceutics · 2020Review
- The miRNA-mRNA interactome of murine induced pluripotent stem cell-derived chondrocytes in response to inflammatory cytokines.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Biological resurfacing of entire articular surfaces represents an important but challenging strategy for treatment of cartilage degeneration that occurs in osteoarthritis. Not only does this approach require anatomically sized and functional engineered cartilage, but the inflammatory environment within an arthritic joint may also inhibit chondrogenesis and induce degradation of native and engineered cartilage. The goal of this study was to use adult stem cells to engineer anatomically shaped, functional cartilage constructs capable of tunable and inducible expression of antiinflammatory molecules, specifically IL-1 receptor antagonist (IL-1Ra). Large (22-mm-diameter) hemispherical scaffolds were fabricated from 3D woven poly(ε-caprolactone) (PCL) fibers into two different configurations and seeded with human adipose-derived stem cells (ASCs). Doxycycline (dox)-inducible lentiviral vectors containing eGFP or IL-1Ra transgenes were immobilized to the PCL to transduce ASCs upon seeding, and constructs were cultured in chondrogenic conditions for 28 d. Constructs showed biomimetic cartilage properties and uniform tissue growth while maintaining their anatomic shape throughout culture. IL-1Ra-expressing constructs produced nearly 1 µg/mL of IL-1Ra upon controlled induction with dox. Treatment with IL-1 significantly increased matrix metalloprotease activity in the conditioned media of eGFP-expressing constructs but not in IL-1Ra-expressing constructs. Our findings show that advanced textile manufacturing combined with scaffold-mediated gene delivery can be used to tissue engineer large anatomically shaped cartilage constructs that possess controlled delivery of anticytokine therapy. Importantly, these cartilage constructs have the potential to provide mechanical functionality immediately upon implantation, as they will need to replace a majority, if not the entire joint surface to restore function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.