ArticleProceedings of the National Academy of Sciences of the United States of America2016
Regulatory role of the respiratory supercomplex factors in Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes.FEBS letters · 2026Article
- Transient protein structure guides surface diffusion pathways for electron transport in membrane supercomplexes.Nature communications · 2026Article
- The functional role of Higd1a in mitochondrial homeostasis and in multiple disease processes.Genes & diseases · 2023Review
- Cryo-EM structure and function of S. pombe complex IV with bound respiratory supercomplex factor.Communications chemistry · 2023Article
- Structure and Mechanism of Respiratory III-IV Supercomplexes in Bioenergetic Membranes.Chemical reviews · 2021Review
- Cryo-EM structure and kinetics reveal electron transfer by 2D diffusion of cytochromeProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Molecular and Supramolecular Structure of the Mitochondrial Oxidative Phosphorylation System: Implications for Pathology.Life (Basel, Switzerland) · 2021Review
- HIGD-Driven Regulation of CytochromeCells · 2020Review
- HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.Molecular & cellular proteomics : MCP · 2020Article
- Rcf2 revealed in cryo-EM structures of hypoxic isoforms of mature mitochondrial III-IV supercomplexes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Structural changes at the surface of cytochrome c oxidase alter the proton-pumping stoichiometry.Biochimica et biophysica acta. Bioenergetics · 2020Article
- Hypoxia-inducible gene domain 1 proteins in yeast mitochondria protect against proton leak through complex IV.The Journal of biological chemistry · 2019Article
- The yeast mitochondrial proteins Rcf1 and Rcf2 support the enzymology of the cytochromeThe Journal of biological chemistry · 2019Article
- Rcf1 Modulates CytochromeFrontiers in physiology · 2019Article
- Control of transmembrane charge transfer in cytochrome c oxidase by the membrane potential.Nature communications · 2018Article
- Regulation of cytochrome c oxidase activity by modulation of the catalytic site.Scientific reports · 2018Article
- Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes.Essays in biochemistry · 2018Review
- Respiratory chain supercomplexes: Structures, function and biogenesis.Seminars in cell & developmental biology · 2018Review
- Solution NMR structure of yeast Rcf1, a protein involved in respiratory supercomplex formation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Streptozotocin-Induced Adaptive Modification of Mitochondrial Supercomplexes in Liver of Wistar Rats and the Protective Effect ofBiochemistry research international · 2018Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The respiratory supercomplex factors (Rcf) 1 and 2 mediate supramolecular interactions between mitochondrial complexes III (ubiquinol-cytochrome c reductase; cyt. bc1) and IV (cytochrome c oxidase; CytcO). In addition, removal of these polypeptides results in decreased activity of CytcO, but not of cyt. bc1 In the present study, we have investigated the kinetics of ligand binding, the single-turnover reaction of CytcO with O2, and the linked cyt. bc1-CytcO quinol oxidation-oxygen-reduction activities in mitochondria in which Rcf1 or Rcf2 were removed genetically (strains rcf1Δ and rcf2Δ, respectively). The data show that in the rcf1Δ and rcf2Δ strains, in a significant fraction of the population, ligand binding occurs over a time scale that is ∼100-fold faster (τ ≅ 100 μs) than observed with the wild-type mitochondria (τ ≅ 10 ms), indicating structural changes. This effect is specific to removal of Rcf and not dissociation of the cyt. bc1-CytcO supercomplex. Furthermore, in the rcf1Δ and rcf2Δ strains, the single-turnover reaction of CytcO with O2 was incomplete. This observation indicates that the lower activity of CytcO is caused by a fraction of inactive CytcO rather than decreased CytcO activity of the entire population. Furthermore, the data suggest that the Rcf1 polypeptide mediates formation of an electron-transfer bridge from cyt. bc1 to CytcO via a tightly bound cyt. c We discuss the significance of the proposed regulatory mechanism of Rcf1 and Rcf2 in the context of supramolecular interactions between cyt. bc1 and CytcO.
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