ArticleProceedings of the National Academy of Sciences of the United States of America2016
Directed evolution of a sphingomyelin flippase reveals mechanism of substrate backbone discrimination by a P4-ATPase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Measuring Phospholipid Flippase Activity by NBD-Lipid Uptake in Living Yeast Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Flipping the script: Advances in understanding how and why P4-ATPases flip lipid across membranes.Biochimica et biophysica acta. Molecular cell research · 2024Review
- Arabidopsis P4 ATPase-mediated cell detoxification confers resistance to Fusarium graminearum and Verticillium dahliae.Nature communications · 2021Article
- P-Type ATPase Apt1 of the Fungal PathogenJournal of fungi (Basel, Switzerland) · 2021Article
- Exofacial membrane composition and lipid metabolism regulates plasma membrane P4-ATPase substrate specificity.The Journal of biological chemistry · 2020Article
- Crystal structure of a human plasma membrane phospholipid flippase.The Journal of biological chemistry · 2020Article
- Conserved mechanism of phospholipid substrate recognition by the P4-ATPase Neo1 from Saccharomyces cerevisiae.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Article
- The PQ-loop protein Any1 segregates Drs2 and Neo1 functions required for viability and plasma membrane phospholipid asymmetry.Journal of lipid research · 2019Article
- Yeast and human P4-ATPases transport glycosphingolipids using conserved structural motifs.The Journal of biological chemistry · 2019Article
- Phospholipid flipping involves a central cavity in P4 ATPases.Scientific reports · 2017Article
- Disease mutations reveal residues critical to the interaction of P4-ATPases with lipid substrates.Scientific reports · 2017Article
- Aminoglycerophospholipid flipping and P4-ATPases in Toxoplasma gondii.The Journal of biological chemistryArticle
- Decoding P4-ATPase substrate interactions.Critical reviews in biochemistry and molecular biologyReview
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2 authors.
Funding
Abstract
Phospholipid flippases in the type IV P-type ATPase (P4-ATPases) family establish membrane asymmetry and play critical roles in vesicular transport, cell polarity, signal transduction, and neurologic development. All characterized P4-ATPases flip glycerophospholipids across the bilayer to the cytosolic leaflet of the membrane, but how these enzymes distinguish glycerophospholipids from sphingolipids is not known. We used a directed evolution approach to examine the molecular mechanisms through which P4-ATPases discriminate substrate backbone. A mutagenesis screen in the yeast Saccharomyces cerevisiae has identified several gain-of-function mutations in the P4-ATPase Dnf1 that facilitate the transport of a novel lipid substrate, sphingomyelin. We found that a highly conserved asparagine (N220) in the first transmembrane segment is a key enforcer of glycerophospholipid selection, and specific substitutions at this site allow transport of sphingomyelin.
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