ArticleThe Journal of biological chemistry2016
An Oral Load of [13C3]Glycerol and Blood NMR Analysis Detect Fatty Acid Esterification, Pentose Phosphate Pathway, and Glycerol Metabolism through the Tricarboxylic Acid Cycle in Human Liver.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03480594 (Effect of Fatty Liver on TCA Cycle Flux and the Pentose Phosphate Pathway), which is not on this map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effect of Fatty Liver on TCA Cycle Flux and the Pentose Phosphate Pathway (HP FFF)
Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Histone H4K5 lactylation-driven feedback loop modulates glycerol-linked immunosuppressive activity of tumor-associated T regulatory cells.Signal transduction and targeted therapy · 2026Article
- Disrupted metabolic flux balance between pyruvate dehydrogenase and pyruvate carboxylase in human fatty liver.Metabolism: clinical and experimental · 2025Article
- The neglected PCK1/glucagon (inter)action in nutrient homeostasis beyond gluconeogenesis: Disease pathogenesis and treatment.Molecular metabolism · 2025Review
- Analyses of mitochondrial metabolism in diseases: a review onRSC advances · 2024Review
- Integration of Liver Glycogen and Triglyceride NMR Isotopomer Analyses Provides a Comprehensive Coverage of Hepatic Glucose and Fructose Metabolism.Metabolites · 2022Article
- Differential Metabolism of Glycerol Based on Oral versus Intravenous Administration in Humans.Metabolites · 2022Article
- A randomized clinical trial evaluating the effect of empagliflozin on triglycerides in obese adults: Role of visceral fat.Metabolism open · 2022Article
- Advances in stable isotope tracer methodology part 1: hepatic metabolism via isotopomer analysis and postprandial lipolysis modeling.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2020Article
- A simple method to monitor hepatic gluconeogenesis and triglyceride synthesis following oral sugar tolerance test in obese adolescents.American journal of physiology. Regulatory, integrative and comparative physiology · 2019Article
- Assessing the pentose phosphate pathway using [2, 3-NMR in biomedicine · 2019Article
- Fatty liver disrupts glycerol metabolism in gluconeogenic and lipogenic pathways in humans.Journal of lipid research · 2018Article
- Pentose phosphate pathway activity parallels lipogenesis but not antioxidant processes in rat liver.American journal of physiology. Endocrinology and metabolism · 2018Article
- tcaSIM: A Simulation Program for Optimal Design ofCurrent Metabolomics · 2018Article
- Effects of visceral adiposity on glycerol pathways in gluconeogenesis.Metabolism: clinical and experimental · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Drugs and other interventions for high impact hepatic diseases often target biochemical pathways such as gluconeogenesis, lipogenesis, or the metabolic response to oxidative stress. However, traditional liver function tests do not provide quantitative data about these pathways. In this study, we developed a simple method to evaluate these processes by NMR analysis of plasma metabolites. Healthy subjects ingested [U-(13)C3]glycerol, and blood was drawn at multiple times. Each subject completed three visits under differing nutritional states. High resolution (13)C NMR spectra of plasma triacylglycerols and glucose provided new insights into a number of hepatic processes including fatty acid esterification, the pentose phosphate pathway, and gluconeogenesis through the tricarboxylic acid cycle. Fasting stimulated pentose phosphate pathway activity and metabolism of [U-(13)C3]glycerol in the tricarboxylic acid cycle prior to gluconeogenesis or glyceroneogenesis. Fatty acid esterification was transient in the fasted state but continuous under fed conditions. We conclude that a simple NMR analysis of blood metabolites provides an important biomarker of pentose phosphate pathway activity, triacylglycerol synthesis, and flux through anaplerotic pathways in mitochondria of human liver.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.