ArticleJournal of proteome research2016
Mass Spectrometric Quantification of N-Linked Glycans by Reference to Exogenous Standards.
Article in Journal of proteome research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Glycosphingolipids and their impact on platelet activity in a murine model of fabry disease.Scientific reports · 2024Article
- Comparison of N-Glycopeptide to Released N-Glycan Abundances and the Influence of Glycopeptide Mass and Charge States on N-Linked Glycosylation of IgG Antibodies.Journal of proteome research · 2024Article
- Neural-specific alterations in glycosphingolipid biosynthesis and cell signaling associated with two human ganglioside GM3 synthase deficiency variants.Human molecular genetics · 2023Article
- Characterization of the human platelet N- and O-glycome upon storage using tandem mass spectrometry.Blood advances · 2023Article
- Comprehensive analysis of platelet glycoprotein Ibα ectodomain glycosylation.Journal of thrombosis and haemostasis : JTH · 2023Article
- Article
- ReliableMolecules (Basel, Switzerland) · 2023Article
- Cystic Fibrosis Airway Mucus Hyperconcentration Produces a Vicious Cycle of Mucin, Pathogen, and Inflammatory Interactions that Promotes Disease Persistence.American journal of respiratory cell and molecular biology · 2022Article
- Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.Science advances · 2022Article
- Quantitative non-targeted analysis: Bridging the gap between contaminant discovery and risk characterization.Environment international · 2022Article
- Platelet and myeloid cell phenotypes in a rat model of Fabry disease.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- A mutation in SLC37A4 causes a dominantly inherited congenital disorder of glycosylation characterized by liver dysfunction.American journal of human genetics · 2021Article
- Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation.Molecular & cellular proteomics : MCP · 2021Review
- Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.Frontiers in molecular biosciences · 2021Article
- Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MSMolecular & cellular proteomics : MCP · 2021Article
- Glycomics-informed glycoproteomic analysis of site-specific glycosylation for SARS-CoV-2 spike protein.STAR protocols · 2020Article
- Mass spectrometric quantification of plasma glycosphingolipids in human GM3 ganglioside deficiency.Clinical mass spectrometry (Del Mar, Calif.) · 2019Article
- Airway glycomic and allergic inflammatory consequences resulting from keratan sulfate galactose 6-O-sulfotransferase (CHST1) deficiency.Glycobiology · 2018Article
- Glucuronylated core 1 glycans are required for precise localization of neuromuscular junctions and normal formation of basement membranes on Drosophila muscles.Developmental biology · 2018Article
- Neuropathic pain in a Fabry disease rat model.JCI insight · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Environmental and metabolic processes shape the profile of glycoprotein glycans expressed by cells, whether in culture, developing tissues, or mature organisms. Quantitative characterization of glycomic changes associated with these conditions has been achieved historically by reductive coupling of oligosaccharides to various fluorophores following release from glycoprotein and subsequent HPLC or capillary electrophoretic separation. Such labeling-based approaches provide a robust means of quantifying glycan amount based on fluorescence yield. Mass spectrometry, on the other hand, has generally been limited to relative quantification in which the contribution of the signal intensity for an individual glycan is expressed as a percent of the signal intensity summed over the total profile. Relative quantification has been valuable for highlighting changes in glycan expression between samples; sensitivity is high, and structural information can be derived by fragmentation. We have investigated whether MS-based glycomics is amenable to absolute quantification by referencing signal intensities to well-characterized oligosaccharide standards. We report the qualification of a set of N-linked oligosaccharide standards by NMR, HPLC, and MS. We also demonstrate the dynamic range, sensitivity, and recovery from complex biological matrices for these standards in their permethylated form. Our results indicate that absolute quantification for MS-based glycomic analysis is reproducible and robust utilizing currently available glycan standards.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.