ArticleScientific reports2016
Yeast metabolic chassis designs for diverse biotechnological products.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Model-guided chemical environment and metabolic network design to couple pathways with cell fitness.Metabolic engineering communications · 2025Article
- Predictive evolution of metabolic phenotypes using model-designed environments.Molecular systems biology · 2022Article
- Standardization of Synthetic Biology Tools and Assembly Methods forACS synthetic biology · 2022Review
- Comparison of the Unfolded Protein Response in Cellobiose Utilization of Recombinant Angel- and W303-1A-Derived Yeast Expressing β-Glucosidase.Frontiers in bioengineering and biotechnology · 2022Article
- Model-guided development of an evolutionarily stable yeast chassis.Molecular systems biology · 2021Article
- Mitochondrial respiration is required to provide amino acids during fermentative proliferation of fission yeast.EMBO reports · 2020Article
- Engineering and modification of microbial chassis for systems and synthetic biology.Synthetic and systems biotechnology · 2019Review
- Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods.Nature communications · 2018Article
- Bespoke design of whole-cell microbial machines.Microbial biotechnology · 2017Article
- Article
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Authors and funding
6 authors.
Funding
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Abstract
The diversity of industrially important molecules for which microbial production routes have been experimentally demonstrated is rapidly increasing. The development of economically viable producer cells is, however, lagging behind, as it requires substantial engineering of the host metabolism. A chassis strain suitable for production of a range of molecules is therefore highly sought after but remains elusive. Here, we propose a genome-scale metabolic modeling approach to design chassis strains of Saccharomyces cerevisiae - a widely used microbial cell factory. For a group of 29 products covering a broad range of biochemistry and applications, we identified modular metabolic engineering strategies for re-routing carbon flux towards the desired product. We find distinct product families with shared targets forming the basis for the corresponding chassis cells. The design strategies include overexpression targets that group products by similarity in precursor and cofactor requirements, as well as gene deletion strategies for growth-product coupling that lead to non-intuitive product groups. Our results reveal the extent and the nature of flux re-routing necessary for producing a diverse range of products in a widely used cell factory and provide blueprints for constructing pre-optimized chassis strains.
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