ArticleScientific reports2016
The Dentin Sialoprotein (DSP) Domain Regulates Dental Mesenchymal Cell Differentiation through a Novel Surface Receptor.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Beyond the diagnosis: UnravelingThe Japanese dental science review · 2026Review
- Reduced Dietary Protein Induces Changes in the Dental Proteome.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2026Article
- Splicing mutation in DSPP causes dentinogenesis imperfecta and amelogenesis imperfecta.BMC oral health · 2026Article
- Dentin Sialophosphoprotein Expression Profile in Developing Human Primary Teeth: An Experimental Study.Journal of dentistry (Shiraz, Iran) · 2025Article
- Review
- Review
- A critical review of in vitro research methodologies used to study mineralization in human dental pulp cell cultures.International endodontic journal · 2022Review
- SMAD4 Feedback Activates the Canonical TGF-β Family Signaling Pathways.International journal of molecular sciences · 2021Article
- Dentine sialophosphoprotein signal in dentineogenesis and dentine regeneration.European cells & materials · 2021Review
- Comparison and Contrast of Bone and Dentin in Genetic Disorder, Morphology and Regeneration: A Review.Journal of bone metabolism · 2021Article
- Article
- Histological Review of Demineralized Dentin Matrix as a Carrier of rhBMP-2.Tissue engineering. Part B, Reviews · 2020Review
- Article
- Gelatinases Cleave Dentin Sialoprotein Intracellularly.Frontiers in physiology · 2020Article
- Intermittent compressive force promotes osteogenic differentiation in human periodontal ligament cells by regulating the transforming growth factor-β pathway.Cell death & disease · 2019Article
- Mechanical strain triggers differentiation of dental mesenchymal stem cells by activating osteogenesis-specific biomarkers expression.American journal of translational research · 2019Article
- Compromised alveolar bone cells in a patient with dentinogenesis imperfecta caused by DSPP mutation.Clinical oral investigations · 2019Article
- The functional significance of dentin sialoprotein-phosphophoryn and dentin sialoprotein.International journal of oral science · 2018Review
- Dentin sialoprotein facilitates dental mesenchymal cell differentiation and dentin formation.Scientific reports · 2017Article
- Dentin Sialoprotein is a Novel Substrate of Matrix Metalloproteinase 9 in vitro and in vivo.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Dentin sialophosphoprotein (DSPP) is a dentin extracellular matrix protein that is processed into dentin sialoprotein (DSP), dentin glycoprotein (DGP) and dentin phosphoprotein (DPP). DSP is mainly expressed in odontoblasts. We hypothesized that DSP interacts with cell surface receptors and subsequently activates intracellular signaling. Using DSP as bait for screening a protein library, we demonstrate that DSP acts as a ligand and binds to integrin β6. The 36 amino acid residues of DSP are sufficient to bind to integrin β6. This peptide promoted cell attachment, migration, differentiation and mineralization of dental mesenchymal cells. In addition, DSP (aa183-219) stimulated phosphorylation of ERK1/2 and P38 kinases. This activation was inhibited by an anti-integrin β6 antibody and siRNA. Furthermore, we demonstrate that this DSP fragment induces SMAD1/5/8 phosphorylation and nuclear translocation via ERK1/2 and P38 signaling. SMAD1/5/8 binds to SMAD binding elements (SBEs) in the DSPP gene promoter. SBE mutations result in a decrease in DSPP transcriptional activity. Endogenous DSPP expression was up-regulated by DSP (aa183-219) in dental mesenchymal cells. The data in the current study demonstrate for the first time that this DSP domain acts as a ligand in a RGD-independent manner and is involved in intracellular signaling via interacting with integrin β6. The DSP domain regulates DSPP expression and odontoblast homeostasis via a positive feedback loop.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.