Evidence map›Paper›PMID 27430310›Full record

ArticleBMC oral health2016

Bone conditioned media (BCM) improves osteoblast adhesion and differentiation on collagen barrier membranes.

Masako Fujioka-Kobayashi, Jordi Caballé-Serrano, Dieter D Bosshardt, Reinhard Gruber, Daniel Buser, Richard J Miron

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC oral health, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
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  5. On the search of the ideal barrier membrane for guided bone regeneration.Journal of clinical and experimental dentistry · 2018
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Masako Fujioka-KobayashiDepartment of Cranio and Maxillofacial Surgery, University of Bern, Bern, Switzerland.
Jordi Caballé-SerranoDepartment of Oral Surgery and Stomatology, Laboratory of Oral Cell Biology, University of Bern, Freiburgstrasse 7, Bern, 3010, Switzerland.
Dieter D BosshardtRobert K. Schenk Laboratory of Oral Histology, University of Bern, Bern, Switzerland.
Reinhard GruberDepartment of Oral Biology, University of Vienna, Vienna, Austria.
Daniel BuserDepartment of Oral Surgery and Stomatology, University of Bern, Bern, Switzerland.
Richard J MironDepartment of Oral Surgery and Stomatology, Laboratory of Oral Cell Biology, University of Bern, Freiburgstrasse 7, Bern, 3010, Switzerland. richard.miron@zmk.unibe.ch.
University of Bern · CHNova Southeastern University · USUniversity of Vienna · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe use of autogenous bone chips during guided bone regeneration procedures has remained the gold standard for bone grafting due to its excellent combination of osteoconduction, osteoinduction and osteogenesis. Recent protocols established by our group have characterized specific growth factors and cytokines released from autogenous bone that have the potential to be harvested and isolated into bone conditioned media (BCM). Due to the advantageous osteo-promotive properties of BCM, the aims of the present study was to pre-coat collagen barrier membranes with BCM and investigate its effect on osteoblast adhesion, proliferation and differentiation for possible future clinical use.

methodsScanning electron microscopy (SEM) was first used to qualitative assess BCM protein accumulation on the surface of collagen membranes. Thereafter, undifferentiated mouse ST2 stromal bone marrow cells were seeded onto BioGide porcine derived collagen barrier membranes (control) or barrier membranes pre-coated with BCM (test group). Control and BCM samples were compared for cell adhesion at 8 h, cell proliferation at 1, 3 and 5 days and real-time PCR at 5 days for osteoblast differentiation markers including Runx2, alkaline phosphatase (ALP), osteocalcin (OCN) and bone sialoprotein (BSP). Mineralization was further assessed with alizarin red staining at 14 days post seeding.

resultsSEM images demonstrated evidence of accumulated proteins found on the surface of collagen membranes following coating with BCM. Analysis of total cell numbers revealed that the additional pre-coating with BCM markedly increased cell attachment over 4 fold when compared to cells seeded on barrier membranes alone. No significant difference could be observed for cell proliferation at all time points. BCM significantly increased mRNA levels of osteoblast differentiation markers including ALP, OCN and BSP at 5 days post seeding. Furthermore, barrier membranes pre-coated with BCM demonstrated a 5-fold increase in alizarin red staining at 14 days.

conclusionThe results from the present study suggest that the osteoconductive properties of porcine-derived barrier membranes could be further improved by BCM by significantly increasing cell attachment, differentiation and mineralization of osteoblasts in vitro. Future animal testing is required to fully characterize the additional benefits of BCM for guided bone regeneration.

Indexed as

CollagenCulture Media, ConditionedOsteoblastsOsteogenesisAlkaline PhosphataseAnimalsBone and BonesCell DifferentiationCell ProliferationCells, CulturedSwineAlkaline PhosphataseCollagenCulture Media, ConditionedBarrier membranesBone conditioned mediaBone graftingGrowth factorsGuided bone regeneration

Identifiers

PMID27430310
PMCPMC4948089
OpenAlexW2499540508

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.