ArticleInternational journal of molecular sciences2016
Effect of Medium Supplements on Agrobacterium rhizogenes Mediated Hairy Root Induction from the Callus Tissues of Camellia sinensis var. sinensis.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 38 citations in OpenAlex.
- Optimized protocol for efficient generation, confirmation, transformation, and CRISPR editing of grapevine hairy roots.Journal of experimental botany · 2026Article
- Integrative Analysis of Transcriptomic and Metabolomic Profiles Uncovers the Mechanism of Color Variation in the Tea Plant Callus.Plants (Basel, Switzerland) · 2025Article
- A Plant Model ofApplied biosciences · 2024Article
- A Versatile Protocol for Efficient Transformation and Regeneration in MegaMethods and protocols · 2023Article
- Understanding the Origin and Evolution of Tea (Camellia sinensis [L.]): Genomic Advances in Tea.Journal of molecular evolution · 2023Review
- Review
- Conservation of anFrontiers in plant science · 2022Article
- Pretreatment and posttreatment in the biolistic transformation of tea plant (Plant biotechnology (Tokyo, Japan) · 2020Article
- Tea plant genomics: achievements, challenges and perspectives.Horticulture research · 2020Review
- Influence of media supplements on inhibition of oxidative browning and bacterial endophytes of3 Biotech · 2018Article
- Protocol: high-efficiencyPlant methods · 2018Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tea (Camellia sinensis L.) is recalcitrant to Agrobacterium-mediated genetic transformation largely due to the bactericidal effects of tea polyphenols and phenolics oxidation induced by necrosis of explant tissue over the process of transformation. In this study, different antioxidants/adsorbents were added as supplements to the co-cultivation and post co-cultivation media to overcome these problems for the transformation improvement. Tea-cotyledon-derived calli were used as explants and Agrobacterium rhizognes strain ATCC 15834 was used as a mediator. Results showed that Agrobacterium growth, virulence (vir) gene expression and browning of explant tissue were greatly influenced by different supplements. Murashige and Skoog (MS) basal salts medium supplemented with 30 g·L(-1) sucrose, 0.1 g·L(-1) l-glutamine and 5 g·L(-1) polyvinylpolypyrrolidone (PVPP) as co-cultivation and post co-cultivation media could maintain these parameters better that ultimately led to significant improvement of hairy root generation efficiency compared to that in the control (MS + 30 g·L(-1) sucrose). Additionally, the reporter genes β-glucuronidase (gusA) and cyan fluorescent protein (cfp) were also stably expressed in the transgenic hairy roots. Our study would be helpful in establishing a feasible approach for tea biological studies and genetic improvement of tea varieties.
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