ArticleBiomacromolecules2016
Substrate Stiffness Combined with Hepatocyte Growth Factor Modulates Endothelial Cell Behavior.
Article in Biomacromolecules, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
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- Mechanical strategies to promote vascularization for tissue engineering and regenerative medicine.Burns & trauma · 2024Review
- Substrate Stiffness of Bone Microenvironment Controls Functions of Pre-Osteoblasts and Fibroblasts In Vitro.Biomimetics (Basel, Switzerland) · 2023Article
- Interpenetrating network design of bioactive hydrogel coatings with enhanced damage resistance.Journal of materials chemistry. B · 2023Article
- Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix.Biointerphases · 2023Article
- Biomedical polymers: synthesis, properties, and applications.Science China. Chemistry · 2022Review
- miR-22 eluting cardiovascular stent based on a self-healable spongy coating inhibits in-stent restenosis.Bioactive materials · 2021Article
- Substrate stiffness differentially impacts autophagy of endothelial cells and smooth muscle cells.Bioactive materials · 2021Article
- Use of Electrospun Phenylalanine/Poly-ε-Caprolactone Chiral Hybrid Scaffolds to Promote Endothelial Remodeling.Frontiers in bioengineering and biotechnology · 2021Article
- Stiffness Sensing by Cells.Physiological reviews · 2020Review
- Hierarchical Capillary Coating to Biofunctionlize Drug-Eluting Stent for Improving Endothelium Regeneration.Research (Washington, D.C.) · 2020Article
- [Construction of controllable polyethylene glycol bioactive coating with hemocompatibility from the surface of modified glass substrate].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2019Article
- Discrete Subaortic Stenosis: Perspective Roadmap to a Complex Disease.Frontiers in cardiovascular medicine · 2018Article
- Surface engineering for lymphocyte programming.Advanced drug delivery reviews · 2017Review
- Horizontal alignment of 5' -> 3' intergene distance segment tropy with respect to the gene as the conserved basis for DNA transcription.Future science OA · 2017Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Endothelial cells (ECs) play a crucial role in regulating various physiological and pathological processes. The behavior of ECs is modulated by physical (e.g., substrate stiffness) and biochemical cues (e.g., growth factors). However, the synergistic influence of these cues on EC behavior has rarely been investigated. In this study, we constructed poly(l-lysine)/hyaluronan (PLL/HA) multilayer films with different stiffness and exposed ECs to these substrates with and without hepatocyte growth factor (HGF)-supplemented culture medium. We demonstrated that EC adhesion, migration, and proliferation were positively correlated with substrate stiffness and that these behaviors were further promoted by HGF. Interestingly, ECs on the lower stiffness substrates showed stronger responses to HGF in terms of migration and proliferation, suggesting that HGF can profoundly influence stiffness-dependent EC behavior correlated with EC growth. After the formation of an EC monolayer, EC behaviors correlated with endothelial function were evaluated by characterizing monolayer integrity, nitric oxide production, and gene expression of endothelial nitric oxide synthase. For the first time, we demonstrated that endothelial function displayed a negative correlation with substrate stiffness. Although HGF improved endothelial function, HGF was not able to change the stiffness-dependent manner of endothelial functions. Taken together, this study provides insights into the synergetic influence of physical and biochemical cues on EC behavior and offers great potential in the development of optimized biomaterials for EC-based regenerative medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.