ArticleStem cell reports2016
Development of Gonadotropin-Releasing Hormone-Secreting Neurons from Human Pluripotent Stem Cells.
Article in Stem cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- CRISPR activation of DLX5 drives neural progenitors to the GnRH cell fate.Journal of molecular endocrinology · 2026Article
- Involvement of Pax6 in the transcriptional regulation of gnrh1 in the Asian stinging catfish Heteropneustes fossilis.Fish physiology and biochemistry · 2026Article
- NGF administration is associated with increased GnRH immunoreactivity and a GnRH-associated phenotype in hypothalamic NSCs of aging mice.Frontiers in endocrinology · 2026Article
- Dual Role of DLK1 in GnRH Neuron Ontogeny.Stem cell reviews and reports · 2025Article
- An optimized method for directed differentiation of hypothalamic neural stem cells in a 3D culture system.Scientific reports · 2025Article
- Deletion in RMST lncRNA impairs hypothalamic neuronal development in a human stem cell-based model of Kallmann Syndrome.Cell death discovery · 2024Article
- A splice site variant in MADD affects hormone expression in pancreatic β cells and pituitary gonadotropes.JCI insight · 2024Article
- HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells.Genome biology · 2024Article
- In vitro modeling of cranial placode differentiation: Recent advances, challenges, and perspectives.Developmental biology · 2024Review
- The initiation and maintenance of gonadotropin-releasing hormone neuron identity in congenital hypogonadotropic hypogonadism.Frontiers in endocrinology · 2023Review
- Deciphering the Transcriptional Landscape of Human Pluripotent Stem Cell-Derived GnRH Neurons: The Role of Wnt Signaling in Patterning the Neural Fate.Stem cells (Dayton, Ohio) · 2022Article
- A Molecular Analysis of Neural Olfactory Placode Differentiation in Human Pluripotent Stem Cells.Stem cells and development · 2022Article
- FGF8-FGFR1 signaling regulates human GnRH neuron differentiation in a time- and dose-dependent manner.Disease models & mechanisms · 2022Article
- Hidden 'pit'falls in deciphering the gonadotropin releasing hormone neuroendocrine cell lineage.Journal of neuroendocrinology · 2021Review
- Physiological Characterization and Transcriptomic Properties of GnRH Neurons Derived From Human Stem Cells.Endocrinology · 2021Article
- Dopamine-induced interactions of female mouse hypothalamic proteins with progestin receptor-A in the absence of hormone.Journal of neuroendocrinology · 2020Article
- Characterization of the human GnRH neuron developmental transcriptome using aDisease models & mechanisms · 2020Article
- Biosensors for Studies on Adhesion-Mediated Cellular Responses to Their Microenvironment.Frontiers in bioengineering and biotechnology · 2020Review
- Evolutionary Conservation of MKRN3 and Other Makorins and Their Roles in Puberty Initiation and Endocrine Functions.Seminars in reproductive medicine · 2019Review
- Nasal Placode Development, GnRH Neuronal Migration and Kallmann Syndrome.Frontiers in cell and developmental biology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gonadotropin-releasing hormone (GnRH) neurons regulate human puberty and reproduction. Modeling their development and function in vitro would be of interest for both basic research and clinical translation. Here, we report a three-step protocol to differentiate human pluripotent stem cells (hPSCs) into GnRH-secreting neurons. Firstly, hPSCs were differentiated to FOXG1, EMX2, and PAX6 expressing anterior neural progenitor cells (NPCs) by dual SMAD inhibition. Secondly, NPCs were treated for 10 days with FGF8, which is a key ligand implicated in GnRH neuron ontogeny, and finally, the cells were matured with Notch inhibitor to bipolar TUJ1-positive neurons that robustly expressed GNRH1 and secreted GnRH decapeptide into the culture medium. The protocol was reproducible both in human embryonic stem cells and induced pluripotent stem cells, and thus provides a translational tool for investigating the mechanisms of human puberty and its disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.