ArticleDevelopmental biology2016
Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin.
Article in Developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Microbial communities experienced during early development shape the host immune system and epigenome.iScience · 2025Article
- Collagen processing is essential for germ cell identity.Biology open · 2025Article
- Functional annotation of a hugely expanded nanos repertoire in Lytechinus variegatus, the green sea urchin.Molecular reproduction and development · 2023Article
- Article
- CRISPR/Cas9 knockin methodology for the sea urchin embryo.Molecular reproduction and development · 2023Article
- Somatic cell conversion to a germ cell lineage: A violation or a revelation?Journal of experimental zoology. Part B, Molecular and developmental evolution · 2021Review
- CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin.Developmental biology · 2021Article
- Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization.Developmental cell · 2021Article
- Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.Developmental biology · 2019Article
- Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus.International journal of molecular sciences · 2019Article
- Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs).Methods in cell biology · 2019Review
- A quiet space during rush hour: Quiescence in primordial germ cells.Stem cell research · 2017Article
- Function of Nanos1 gene in the development of reproductive organs of Schistosoma japonicum.Parasitology research · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nanos is a translational regulator required for the survival and maintenance of primordial germ cells. In the sea urchin, Strongylocentrotus purpuratus (Sp), Nanos 2 mRNA is broadly transcribed but accumulates specifically in the small micromere (sMic) lineage, in part because of the 3'UTR element GNARLE leads to turnover in somatic cells but retention in the sMics. Here we found that the Nanos 2 protein is also selectively stabilized; it is initially translated throughout the embryo but turned over in the future somatic cells and retained only in the sMics, the future germ line in this animal. This differential stability of Nanos protein is dependent on the open reading frame (ORF), and is independent of the sumoylation and ubiquitylation pathways. Manipulation of the ORF indicates that 68 amino acids in the N terminus of the Nanos protein are essential for its stability in the sMics whereas a 45 amino acid element adjacent to the zinc fingers targets its degradation. Further, this regulation of Nanos protein is cell autonomous, following formation of the germ line. These results are paradigmatic for the unique presence of Nanos in the germ line by a combination of selective RNA retention, distinctive translational control mechanisms (Oulhen et al., 2013), and now also by defined Nanos protein stability.
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