ArticleJournal of industrial microbiology & biotechnology2016
Characterization of an evolved carotenoids hyper-producer of Saccharomyces cerevisiae through bioreactor parameter optimization and Raman spectroscopy.
Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 34 citations in OpenAlex.
- Cystofilobasidium ferigula co-production of lipids and carotenoids using crude glycerol.NPJ science of food · 2026Article
- Production of β-carotene from methanol via rDNA-targeted multi-gene integration and selection with increasing antibiotic concentrations in Komagataella phaffii.World journal of microbiology & biotechnology · 2026Article
- Application of vibrational spectroscopies as process analytical techniques for monitoring fermentation and the conversion of lignocellulosic biomass by oleaginous filamentous fungi.Microbial cell factories · 2025Article
- Induction of point and structural mutations in engineered yeast Saccharomyces cerevisiae improve carotenoid production.World journal of microbiology & biotechnology · 2024Article
- Development of a metabolic engineering technology to simultaneously suppress the expression of multiple genes in yeast and application in carotenoid production.World journal of microbiology & biotechnology · 2024Article
- Arts, cultural heritage, sciences, and micro-/bio-/technology: Impact of biomaterials and biocolorants from antiquity till today!Journal of industrial microbiology & biotechnology · 2024Review
- Industrially Important Fungal Carotenoids: Advancements in Biotechnological Production and Extraction.Journal of fungi (Basel, Switzerland) · 2023Review
- Elevated β-Carotene Production Using Codon-Adapted CarRA&B and Metabolic Balance in EngineeredFrontiers in microbiology · 2021Article
- Article
- EngineeringFrontiers in bioengineering and biotechnology · 2020Article
- Beneficial mutations for carotenoid production identified from laboratory-evolved Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2019Article
- Build Your Bioprocess on a Solid Strain-β-Carotene Production in RecombinantFrontiers in bioengineering and biotechnology · 2019Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An evolutionary engineering approach for enhancing heterologous carotenoids production in an engineered Saccharomyces cerevisiae strain was used previously to isolate several carotenoids hyper-producers from the evolved populations. β-Carotene production was characterized in the parental and one of the evolved carotenoids hyper-producers (SM14) using bench-top bioreactors to assess the impact of pH, aeration, and media composition on β-carotene production levels. The results show that with maintaining a low pH and increasing the carbon-to-nitrogen ratio (C:N) from 8.8 to 50 in standard YNB medium, a higher β-carotene production level at 25.52 ± 2.15 mg β-carotene g(-1) (dry cell weight) in the carotenoids hyper-producer was obtained. The increase in C:N ratio also significantly increased carotenoids production in the parental strain by 298 % [from 5.68 ± 1.24 to 22.58 ± 0.11 mg β-carotene g(-1) (dcw)]. In this study, it was shown that Raman spectroscopy is capable of monitoring β-carotene production in these cultures. Raman spectroscopy is adaptable to large-scale fermentations and can give results in near real-time. Furthermore, we found that Raman spectroscopy was also able to measure the relative lipid compositions and protein content of the parental and SM14 strains at two different C:N ratios in the bioreactor. The Raman analysis showed a higher total fatty acid content in the SM14 compared with the parental strain and that an increased C:N ratio resulted in significant increase in total fatty acid content of both strains. The data suggest a positive correlation between the yield of β-carotene per biomass and total fatty acid content of the cell.
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