Evidence map›Paper›PMID 27423862›Full record

ArticleMethods in enzymology2016

An IF-FISH Approach for Covisualization of Gene Loci and Nuclear Architecture in Fission Yeast.

K-D Kim, O Iwasaki, K Noma

Open access · greenAbstract read
In one paragraph

Article in Methods in enzymology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Ferrotoxicity and Its Amelioration by Calcitriol in Cultured Renal Cells.Analytical cellular pathology (Amsterdam) · 2021
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

K-D KimThe Wistar Institute, Philadelphia, PA, United States.
O IwasakiThe Wistar Institute, Philadelphia, PA, United States.
K NomaThe Wistar Institute, Philadelphia, PA, United States. Electronic address: noma@wistar.org.
The Wistar Institute · US

Funding

Tumor Microenvironment and MetastasisP30CA010815 · NCI · WISTAR INSTITUTE · PI Alessandro Gardini · 1985 to 2026
$75.9M
A New Methodology to Decipher Three-Dimensional Genome StructureDP2OD004348 · OD · WISTAR INSTITUTE · PI NOMA, KENICHI · 2008 to 2008
$3.1M
NCI NIH HHS P30 CA010815NIH HHS DP2 OD004348
6 · The paper itself

Abstract

Recent genomic studies have revealed that chromosomal structures are formed by a hierarchy of organizing processes ranging from gene associations, including interactions among enhancers and promoters, to topologically associating domain formations. Gene associations identified by these studies can be characterized by microscopic analyses. Fission yeast is a model organism, in which gene associations have been broadly mapped across the genome, although many of those associations have not been further examined by cell biological approaches. To address the technically challenging process of the visualization of associating gene loci in the fission yeast nuclei, we provide, in detail, an IF-FISH procedure that allows for covisualizing both gene loci and nuclear structural markers such as the nuclear membrane and nucleolus.

Indexed as

Chromosomes, FungalGenetic LociCentromereFluorescent Antibody TechniqueGenome, FungalIn Situ Hybridization, FluorescenceSchizosaccharomycesFISHFission yeastGene positioningGenome organizationIFNuclear architectureNuclear membraneNucleolus

Identifiers

PMID27423862
PMCPMC5189976
OpenAlexW2409739164

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.