ArticleJournal of assisted reproduction and genetics2016
Reanalysis of human blastocysts with different molecular genetic screening platforms reveals significant discordance in ploidy status.
Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- PGT-A is associated with reduced cumulative live birth rate in first reported IVF stimulation cycles age ≤ 40: an analysis of 133,494 autologous cycles reported to SART CORS.Journal of assisted reproduction and genetics · 2023Article
- From contemplation to classification of chromosomal mosaicism in human preimplantation embryos.Journal of assisted reproduction and genetics · 2021Review
- The 2019 PGDIS position statement on transfer of mosaic embryos within a context of new information on PGT-A.Reproductive biology and endocrinology : RB&E · 2020Article
- The mechanisms and clinical application of mosaicism in preimplantation embryos.Journal of assisted reproduction and genetics · 2020Review
- Re-analysis of whole blastocysts after trophectoderm biopsy indicated chromosome aneuploidy.Human genomics · 2020Article
- Should We Offer In Vitro Fertilization to Couples with Unexplained Recurrent Pregnancy Loss?Journal of clinical medicine · 2019Review
- Current status of spent embryo media research for preimplantation genetic testing.Journal of assisted reproduction and genetics · 2019Review
- Preimplantation Genetic Diagnosis of Neurodegenerative Diseases: Review of Methodologies and Report of Our Experience as a Regional Reference Laboratory.Diagnostics (Basel, Switzerland) · 2019Article
- Novel Double Factor PGT strategy analyzing blastocyst stage embryos in a single NGS procedure.PloS one · 2018Article
- Re-analysis of aneuploidy blastocysts with an inner cell mass and different regional trophectoderm cells.Journal of assisted reproduction and genetics · 2017Article
- Is the hypothesis of preimplantation genetic screening (PGS) still supportable? A review.Journal of ovarian research · 2017Review
- Noninvasive prenatal diagnosis of fetal aneuploidy by circulating fetal nucleated red blood cells and extravillous trophoblasts using silicon-based nanostructured microfluidics.Molecular cytogenetics · 2017Article
- Mosaicism: throwing the baby out with the bath water?Journal of assisted reproduction and genetics · 2017Article
- Should preimplantation genetic screening (PGS) be implemented to routine IVF practice?Journal of assisted reproduction and genetics · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe objective of this study is to determine mosaicism and its effect on blastocysts; abnormal blastocysts determined by molecular testing were sequentially biopsied and retested. MATERIAL AND
methodWe re-biopsied 37 blastocyst-stage abnormal embryos from eight patients, which were reanalyzed to determine the level of concordance between biopsies and inter-laboratory congruence between reputable commercial PGS laboratories.
resultsThe main outcome measures were intra-embryo variation between sequential embryo biopsies and inter-laboratory variation between two PGS laboratories. The compatibility between both aCGH and NGS was found to be 11 % (3/27). Importantly, 9/27 (33 %) of embryos originally reported to be aneuploid, upon repeat assessment, were found to be euploid. The concurrence for SNP array and NGS was 50 % (3/6), and 17 % (1/6) of these abnormal embryos tested normal upon re-evaluation with NGS. NGS resulted 41 % (11/27) normal results when 27 of CGH abnormal embryos were retested. Concordance between aCGH and NGS was 4 % (1/27) whereas in three instances, gender discrepancy was observed with NGS when aCGH abnormal embryos were reanalyzed.
conclusionsThe results of these studies reinforce the prevalence of inconsistencies during PGS evaluation of trophectoderm biopsies possibly due to variations in platform sensitivity and heightening concerns over the clinical tractability of such technology in human ARTs..
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.