ArticleAmerican journal of physiology. Heart and circulatory physiology2016
4D intravital microscopy uncovers critical strain differences for the roles of PECAM and CD99 in leukocyte diapedesis.
Article in American journal of physiology. Heart and circulatory physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Microglia promote vascular remodeling in a mouse model of chronic stress.Brain, behavior, and immunity · 2026Article
- Prostaglandin EThe American journal of pathology · 2026Article
- Toward development of a dynamic supramolecular peptide therapy for acute ischemic stroke.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2026Article
- PMN recruitment in inflammatory lung injury models follows classical transendothelial migration paradigms requiring PECAM-1 and CD99.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- The diverse roles of neutrophils from protection to pathogenesis.Nature immunology · 2024Review
- Age-related loss of Notch3 underlies brain vascular contractility deficiencies, glymphatic dysfunction, and neurodegeneration in mice.The Journal of clinical investigation · 2024Article
- Mechanotransduction via endothelial adhesion molecule CD31 initiates transmigration and reveals a role for VEGFR2 in diapedesis.Immunity · 2023Article
- Human CD99L2 Regulates a Unique Step in Leukocyte Transmigration.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Spatiotemporal restriction of endothelial cell calcium signaling is required during leukocyte transmigration.The Journal of experimental medicine · 2021Article
- Dysregulation of Leukocyte Trafficking in Type 2 Diabetes: Mechanisms and Potential Therapeutic Avenues.Frontiers in cell and developmental biology · 2021Review
- Released Myeloperoxidase Attenuates Neutrophil Migration and Accumulation in Inflamed Tissue.Frontiers in immunology · 2021Article
- Clinical and preclinical characterization of CD99 isoforms in acute myeloid leukemia.Haematologica · 2020Article
- Endothelial IQGAP1 regulates leukocyte transmigration by directing the LBRC to the site of diapedesis.The Journal of experimental medicine · 2019Article
- Endothelial cell-specific anticoagulation reduces inflammation in a mouse model of acute lung injury.Acta pharmacologica Sinica · 2019Article
- Review
- PECAM-1 Stabilizes Blood-Brain Barrier Integrity and Favors Paracellular T-Cell Diapedesis Across the Blood-Brain Barrier During Neuroinflammation.Frontiers in immunology · 2019Article
- In vivo imaging reveals unique neutrophil transendothelial migration patterns in inflamed intestines.Mucosal immunology · 2018Article
- More Than Just a Removal Service: Scavenger Receptors in Leukocyte Trafficking.Frontiers in immunology · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Leukocyte transendothelial migration (TEM) is an essential component of the inflammatory response. In vitro studies with human cells have demonstrated that platelet/endothelial cell adhesion molecule (PECAM) functions upstream of CD99 during TEM; however, results in vivo with mice have been apparently contradictory. In this study we use four-dimensional (4D) intravital microscopy to demonstrate that the site and order of function of PECAM and CD99 in vivo are dependent on the strain of mice. In FVB/n mice, PECAM functions upstream of CD99, as in human cells in vitro, and blocking antibodies against either molecule arrest neutrophils before they traverse the endothelium. However, in C57BL/6 mice, PECAM and CD99 appear to function at a different step, as the same antibodies arrest leukocyte migration through the endothelial basement membrane. These results are the first direct comparison of PECAM and CD99 function in different murine strains as well as the first demonstration of the sequential function of PECAM and CD99 in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.