ArticleBiology open2016
Loss of function of the Drosophila Ninein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development.
Article in Biology open, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Review
- Ninein isoform contributions to intracellular processes and macrophage immune function.The Journal of biological chemistry · 2025Article
- Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization.Molecular biology of the cell · 2024Article
- Article
- Immune system modulation & virus transmission during parasitism identified by multi-species transcriptomics of a declining insect biocontrol system.BMC genomics · 2024Article
- PTRN-1 (CAMSAP) and NOCA-2 (NINEIN) are required for microtubule polarity in Caenorhabditis elegans dendrites.PLoS biology · 2022Article
- Subcellular spatial transcriptomics identifies three mechanistically different classes of localizing RNAs.Nature communications · 2022Article
- Microtubule Anchoring: Attaching Dynamic Polymers to Cellular Structures.Frontiers in cell and developmental biology · 2022Review
- A centriole's subdistal appendages: contributions to cell division, ciliogenesis and differentiation.Open biology · 2021Review
- Human NINEIN polymorphism at codon 1111 is associated with the risk of colorectal cancer.Biomedical reports · 2020Article
- Quantitative analysis of subcellular distributions with an open-source, object-based tool.Biology open · 2020Article
- A perinuclear microtubule-organizing centre controls nuclear positioning and basement membrane secretion.Nature cell biology · 2020Article
- Epidermal development requires ninein for spindle orientation and cortical microtubule organization.Life science alliance · 2019Article
- TheThe Journal of cell biology · 2019Article
- Whole Genome Resequencing Reveals Selection Signatures Associated With Important Traits in Ethiopian Indigenous Goat Populations.Frontiers in genetics · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The centrosome-associated proteins Ninein (Nin) and Ninein-like protein (Nlp) play significant roles in microtubule stability, nucleation and anchoring at the centrosome in mammalian cells. Here, we investigate Blastoderm specific gene 25D (Bsg25D), which encodes the only Drosophila protein that is closely related to Nin and Nlp. In early embryos, we find that Bsg25D mRNA and Bsg25D protein are closely associated with centrosomes and astral microtubules. We show that sequences within the coding region and 3'UTR of Bsg25D mRNAs are important for proper localization of this transcript in oogenesis and embryogenesis. Ectopic expression of eGFP-Bsg25D from an unlocalized mRNA disrupts microtubule polarity in mid-oogenesis and compromises the distribution of the axis polarity determinant Gurken. Using total internal reflection fluorescence microscopy, we show that an N-terminal fragment of Bsg25D can bind microtubules in vitro and can move along them, predominantly toward minus-ends. While flies homozygous for a Bsg25D null mutation are viable and fertile, 70% of embryos lacking maternal and zygotic Bsg25D do not hatch and exhibit chromosome segregation defects, as well as detachment of centrosomes from mitotic spindles. We conclude that Bsg25D is a centrosomal protein that, while dispensable for viability, nevertheless helps ensure the integrity of mitotic divisions in Drosophila.
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