ArticleNucleic acids research2016
Insight into G-quadruplex-hemin DNAzyme/RNAzyme: adjacent adenine as the intramolecular species for remarkable enhancement of enzymatic activity.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 224 citations in OpenAlex.
- Synergistic antioxidant and gene supplementation for high-efficacy retinitis pigmentosa therapy.Science advances · 2026Article
- Rational redesign of high-activity G-quadruplex DNAzyme through flanking and looping of nucleobases.Scientific reports · 2026Article
- Impact of NHAnalytical and bioanalytical chemistry · 2026Article
- A Non-G-Quadruplex Hemin Aptamer Forms a Better Peroxidase Mimicking DNAzyme.Chembiochem : a European journal of chemical biology · 2025Article
- Kinetic analysis of catalytic activity of G-quadruplex/hemin DNAzyme with flanking adenine nucleotides.Scientific reports · 2025Article
- Comparative Analysis of Crystal Violet-Binding Aptamers as Potential Cores for Binary Sensors.International journal of molecular sciences · 2025Article
- DNAzyme-based colorimetric biosensor for rapid detection of Shigella flexneri.Scientific reports · 2025Article
- Self-contained G-quadruplex/hemin DNAzyme: a superior ready-made catalyst for in situ imaging analysis.Nucleic acids research · 2025Article
- Article
- Spermine Enhances the Peroxidase Activities of Multimeric Antiparallel G-quadruplex DNAzymes.Biosensors · 2025Article
- Directed evolution of peroxidase DNAzymes by a function-based approach.Biology methods & protocols · 2025Review
- Article
- A label-free DNAzyme-based colorimetric sensor for the detection of Leptospira interrogans.World journal of microbiology & biotechnology · 2024Article
- DNA Catalysis: Design, Function, and Optimization.Molecules (Basel, Switzerland) · 2024Review
- Article
- DNA G-Quadruplexes as Targets for Natural Product Drug Discovery.Engineering (Beijing, China) · 2024Article
- Review
- CRISPR/Cas14 and G-Quadruplex DNAzyme-Driven Biosensor for Paper-Based Colorimetric Detection of African Swine Fever Virus.ACS sensors · 2024Article
- Optimization and Enhancement of the Peroxidase-like Activity of Hemin in Aqueous Solutions of Sodium Dodecylsulfate.ACS omega · 2023Article
- Evaluation of DNA adduct damage using G-quadruplex-based DNAzyme.Bioactive materials · 2023Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G-quadruplex (G4) with stacked G-tetrads structure is able to bind hemin (iron (III)-protoporphyrin IX) to form a unique type of DNAzyme/RNAzyme with peroxidase-mimicking activity, which has been widely employed in multidisciplinary fields. However, its further applications are hampered by its relatively weak activity compared with protein enzymes. Herein, we report a unique intramolecular enhancement effect of the adjacent adenine (EnEAA) at 3' end of G4 core sequences that significantly improves the activity of G4 DNAzymes. Through detailed investigations of the EnEAA, the added 3' adenine was proved to accelerate the compound I formation in catalytic cycle and thus improve the G4 DNAzyme activity. EnEAA was found to be highly dependent on the unprotonated state of the N1 of adenine, substantiating that adenine might function as a general acid-base catalyst. Further adenine analogs analysis supported that both N1 and exocyclic 6-amino groups in adenine played key role in the catalysis. Moreover, we proved that EnEAA was generally applicable for various parallel G-quadruplex structures and even G4 RNAzyme. Our studies implied that adenine might act analogously as the distal histidine in protein peroxidases, which shed light on the fundamental understanding and rational design of G4 DNAzyme/RNAzyme catalysts with enhanced functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.