Evidence map›Paper›PMID 27421732›Full record

ArticleLipids2016

4-Methylzymosterone and Other Intermediates of Sterol Biosynthesis from Yeast Mutants Engineered in the ERG27 Gene Encoding 3-Ketosteroid Reductase.

Terenzio Ferrante, Alessandro Barge, Silvia Taramino, Simonetta Oliaro-Bosso, Gianni Balliano

Abstract read
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In one paragraph

Article in Lipids, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Terenzio FerranteDepartment of Science and Drug Technology, University of Torino, Via P. Giuria 9, 10125, Turin, Italy.
Alessandro BargeDepartment of Science and Drug Technology, University of Torino, Via P. Giuria 9, 10125, Turin, Italy.
Silvia TaraminoDepartment of Science and Drug Technology, University of Torino, Via P. Giuria 9, 10125, Turin, Italy.
Simonetta Oliaro-BossoDepartment of Science and Drug Technology, University of Torino, Via P. Giuria 9, 10125, Turin, Italy. simona.oliaro@unito.it.
Gianni BallianoDepartment of Science and Drug Technology, University of Torino, Via P. Giuria 9, 10125, Turin, Italy. gianni.balliano@unito.it.
University of Turin · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studies in the post-squalene section of sterol biosynthesis may be hampered by the poor availability of authentic standards. The present study used different yeast strains engineered in 3-ketosteroid reductase (Erg27p) to obtain radioactive and non-radioactive intermediates of sterol biosynthesis hardly or not available commercially. Non-radioactive 3-keto 4-monomethyl sterones were purified from non-saponifiable lipids extracted from cells bearing point-mutated 3-ketosteroid reductase. Two strategies were adopted to prepare the radioactive compounds: (1) incubation of cell homogenates of an ERG27-deletant strain with radioactive lanosterol, (2) incubation of growing cells of a strain expressing point-mutated 3-ketosteroid reductase with radioactive acetate. Chemical reduction of both radioactive and non-radioactive 3-keto sterones gave the physiological 3-β OH sterols, as well as the non-physiological 3-α OH isomers. This combined biological and chemical preparation procedure provided otherwise unavailable or hardly available 4-mono-methyl intermediates of sterol biosynthesis, paving the way for research into their roles in physiological and pathological conditions.

Indexed as

Genetic EngineeringMutationOxidoreductasesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSterolsERG27 protein, S cerevisiaeOxidoreductasesSaccharomyces cerevisiae ProteinsSterols3-Ketosteroid reductase4-Methylfecosterone4-MethylzymosteroneEngineered yeast strainsPost-squalene sterol biosynthesis

Identifiers

PMID27421732
OpenAlexW2474947957

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.