ArticleJournal of virological methods2016
Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load.
Article in Journal of virological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Gastrointestinal pathogens in paediatric patients with diarrhoea during the COVID-19 pandemic in Spain: a multicentre molecular-based prospective study.European journal of pediatrics · 2026Article
- Clinical and genomic surveillance of rotavirus causing acute gastroenteritis in the community of Madrid, Spain, 2021-2023: predominance of equine-like G3 strains.Scientific reports · 2026Article
- Epidemiological, clinical and laboratorial features of rotavirus infection in older children and adults: a multicenter hospital-based study in east China.BMC infectious diseases · 2026Article
- One-Year Wastewater-Based Study: Detection and Molecular Typing of rotaviruses, Noroviruses and Enteroviruses in Sewage of Zahedan, Iran.Food and environmental virology · 2025Article
- Detection of Feline Coronavirus Membrane Gene Based on Conventional Revere Transcription-Polymerase Chain Reaction, Nested Reverse Transcription-Polymerase Chain Reaction, and Reverse Transcription-Quantitative Polymerase Chain Reaction: A Comparative Study.International journal of molecular sciences · 2025Article
- Diversity and Potential Cross-Species Transmission of Rotavirus A in Wild Animals in Yunnan, China.Microorganisms · 2025Article
- Correlates of Rotavirus Vaccine Shedding and Seroconversion in a US Cohort of Healthy Infants.The Journal of infectious diseases · 2024Article
- Detection and molecular characterization of major enteric pathogens in calves in central Ethiopia.BMC veterinary research · 2024Article
- Hybrid capture shotgun sequencing detected unexpected viruses in the cerebrospinal fluid of children with acute meningitis and encephalitis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Detection of G3 human-like rotavirus in institutionalized dogs from Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Rotaviruses in Wild Ungulates from Germany, 2019-2022.Microorganisms · 2023Article
- Shotgun metagenomics to investigate unknown viral etiologies of pediatric meningoencephalitis.PloS one · 2023Article
- Rotavirus A in Domestic Pigs and Wild Boars: High Genetic Diversity and Interspecies Transmission.Viruses · 2022Article
- Rotavirus Strain Trends in United States, 2009-2016: Results from the National Rotavirus Strain Surveillance System (NRSSS).Viruses · 2022Review
- Ocurrence of rotavirus and picobirnavirus in wild and exotic avian from amazon forest.PLoS neglected tropical diseases · 2021Article
- A Remarkable Genetic Diversity of Rotavirus A Circulating in Red Fox Population in Croatia.Pathogens (Basel, Switzerland) · 2021Article
- Article
- Fecal Source Tracking in A Wastewater Treatment and Reclamation System Using Multiple Waterborne Gastroenteritis Viruses.Pathogens (Basel, Switzerland) · 2019Article
- Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon.PloS one · 2018Article
- Molecular and phylogenetic characterization of bovine coronavirus virus isolated from dairy cattle in Central Region, Thailand.Tropical animal health and production · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Techniques such as the real-time reverse transcription-polymerase chain reaction (qRT-PCR) can detect RNA in samples with a low viral load. However, these amplicons typically are either too short or at insufficient concentrations for use in subsequent sequencing reactions for genotyping and detection confirmation. The assay developed in this study detects rotavirus G genotypes and P genotypes with viral loads as low as 6.2 and 8.2 copies per reaction, respectively. The assay was validated using a panel of 91 stool samples, 32 reference rotavirus strains, and 6 non-target enteric virus samples.
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