Evidence map›Paper›PMID 27421133›Full record

ArticleOncotarget2016

CEACAM6 is upregulated by Helicobacter pylori CagA and is a biomarker for early gastric cancer.

Rony K Roy, Michal M Hoppe, Supriya Srivastava, Animesh Samanta, Neel Sharma, Kar Tong Tan, Henry Yang, Dominic C Voon, Brendan Pang, Ming Teh and 11 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.

  1. Pooled it
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  7. Review
  8. The emerging roles of CEACAM6 in human cancer (Review).International journal of oncology · 2024
    Review
  9. Identification of Recurrence-Related mRNAs and Noncoding RNAs in Hepatocellular Carcinoma Following Liver Transplantation.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 4 institutions in 2 countries.

Rony K RoyCancer Science Institute of Singapore, National University of Singapore, Singapore.
Michal M HoppeCancer Science Institute of Singapore, National University of Singapore, Singapore.
Supriya SrivastavaCancer Science Institute of Singapore, National University of Singapore, Singapore.
Animesh SamantaDepartment of Chemistry, National University of Singapore, Singapore.
Neel SharmaDivision of Gastroenterology and Hepatology, National University Hospital, Singapore.
Kar Tong TanCancer Science Institute of Singapore, National University of Singapore, Singapore.
Henry YangCancer Science Institute of Singapore, National University of Singapore, Singapore.
Dominic C VoonCancer Science Institute of Singapore, National University of Singapore, Singapore.
Brendan PangDepartment of Pathology, National University Hospital, Singapore.
Ming TehDepartment of Pathology, National University Hospital, Singapore.
Naoko Murata-KamiyaUniversity of Tokyo, Tokyo, Japan.
Masanori HatakeyamaUniversity of Tokyo, Tokyo, Japan.
Young-Tae ChangDepartment of Chemistry, National University of Singapore, Singapore.
Wei Peng YongCancer Science Institute of Singapore, National University of Singapore, Singapore.
Yoshiaki ItoCancer Science Institute of Singapore, National University of Singapore, Singapore.
Khek Yu HoDepartment of Chemistry, National University of Singapore, Singapore.
Patrick TanCancer Science Institute of Singapore, National University of Singapore, Singapore.
Richie SoongCancer Science Institute of Singapore, National University of Singapore, Singapore.
Phillip H KoefflerCancer Science Institute of Singapore, National University of Singapore, Singapore.
Khay Guan YeohDepartment of Chemistry, National University of Singapore, Singapore.
Anand D JeyasekharanCancer Science Institute of Singapore, National University of Singapore, Singapore.
National University of Singapore · SGNational University Cancer Institute, Singapore · SGNational University Hospital · SGThe University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Early detection of gastric cancers saves lives, but remains a diagnostic challenge. In this study, we aimed to identify cell-surface biomarkers of early gastric cancer. We hypothesized that a subset of plasma membrane proteins induced by the Helicobacter pylori oncoprotein CagA will be retained in early gastric cancers through non-oncogene addiction. An inducible system for expression of CagA was used to identify differentially upregulated membrane protein transcripts in vitro. The top hits were then analyzed in gene expression datasets comparing transcriptome of gastric cancer with normal tissue, to focus on markers retained in cancer. Among the transcripts enriched upon CagA induction in vitro, a significant elevation of CEACAM6 was noted in gene expression datasets of gastric cancer. We used quantitative digital immunohistochemistry to measure CEACAM6 protein levels in tissue microarrays of gastric cancer. We demonstrate an increase in CEACAM6 in early gastric cancers, when compared to matched normal tissue, with an AUC of 0.83 for diagnostic validity. Finally, we show that a fluorescently conjugated CEACAM6 antibody binds avidly to freshly resected gastric cancer xenograft samples and can be detected by endoscopy in real time. Together, these results suggest that CEACAM6 upregulation is a cell surface response to H. pylori CagA, and is retained in early gastric cancers. They highlight a novel link between CEACAM6 expression and CagA in gastric cancer, and suggest CEACAM6 to be a promising biomarker to aid with the fluorescent endoscopic diagnosis of early neoplastic lesions in the stomach.

Indexed as

AnimalsAntigens, BacterialAntigens, CDBacterial ProteinsBiomarkers, TumorCell Adhesion MoleculesFluorescent Antibody TechniqueGPI-Linked ProteinsHelicobacter InfectionsHumansMiceStomach NeoplasmsUp-RegulationAntigens, BacterialAntigens, CDBacterial ProteinsBiomarkers, TumorcagA protein, Helicobacter pyloriCEACAM6 protein, humanCell Adhesion MoleculesGPI-Linked ProteinsbiomarkerCEACAM6endoscopygastric cancerHelicobacter pylori

Identifiers

PMID27421133
PMCPMC5342417
OpenAlexW2463329699

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.