ReviewMicrobial biotechnology2016
A roadmap for biocatalysis - functional and spatial orchestration of enzyme cascades.
Review in Microbial biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed.
- Biocatalytic Arene Functionalization Using Integrated Unspecific Peroxygenase and Halohydrin Dehalogenase.Chem & bio engineering · 2026Article
- A high-sensitivity strategy to screen NAD(P)H-dependent reductase activity by coupled enzyme cascade.The Journal of biological chemistry · 2026Article
- Cell-type-specific alkaloid and terpenoid biosynthesis in glandular trichomes: single-cell and spatial transcriptomic perspectives.Frontiers in plant science · 2026Review
- Expanding the Repertoire of Photoswitchable Unnatural Amino Acids for Enzyme Engineering.Angewandte Chemie (International ed. in English) · 2025Article
- Biomimetic Chemocatalytic Cascades-A New Strategy for Molecular Design of Degradable Polymer Systems.Macromolecules · 2025Article
- Up- and Down-Regulation of Enzyme Activity in Aggregates with Gold-Covered Magnetic Nanoparticles Triggered by Low-Frequency Magnetic Field.Nanomaterials (Basel, Switzerland) · 2024Article
- Tunable T7 Promoter Orthogonality on T7RNAP forACS synthetic biology · 2023Article
- Exploring the Feasibility of Cell-Free Synthesis as a Platform for Polyhydroxyalkanoate (PHA) Production: Opportunities and Challenges.Polymers · 2023Review
- Multienzyme Coimmobilization on Triheterofunctional Supports.Biomacromolecules · 2023Article
- Review
- Oxygenating Biocatalysts for Hydroxyl Functionalisation in Drug Discovery and Development.ChemMedChem · 2022Review
- Controlling Enzymatic Activity by Modulating the Oligomerization State via Chemical Rescue and Optical Control.Chembiochem : a European journal of chemical biology · 2022Article
- Modulation of Transaminase Activity by Encapsulation in Temperature-Sensitive Poly(N-acryloyl glycinamide) Hydrogels.Chembiochem : a European journal of chemical biology · 2021Article
- Engineering analysis of multienzyme cascade reactions for 3'-sialyllactose synthesis.Biotechnology and bioengineering · 2021Article
- Clustering of catalytic nanocompartments for enhancing an extracellular non-native cascade reaction.Chemical science · 2021Article
- Responsive Emulsions for Sequential Multienzyme Cascades.Angewandte Chemie (International ed. in English) · 2021Article
- Plant cell wall architecture guided design of CBM3-GH11 chimeras with enhanced xylanase activity using a tandem repeat left-handed β-3-prism scaffold.Computational and structural biotechnology journal · 2021Article
- Enzymatic synthesis of myricetin 3-Chinese herbal medicines · 2020Article
- Artificial Light-Harvesting Complexes Enable Rieske Oxygenase Catalyzed Hydroxylations in Non-Photosynthetic cells.Angewandte Chemie (International ed. in English) · 2020Article
- Light Regulation of Enzyme Allostery through Photo-responsive Unnatural Amino Acids.Cell chemical biology · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in biological engineering and systems biology have provided new approaches and tools for the industrialization of biology. In the next decade, advanced biocatalytic systems will increasingly be used for the production of chemicals that cannot be made by current processes and/or where the use of enzyme catalysts is more resource efficient with a much reduced environmental impact. We expect that in the future, manufacture of chemicals and materials will utilize both biocatalytic and chemical synthesis synergistically. The realization of such advanced biomanufacturing processes currently faces a number of major challenges. Ready-to-deploy portfolios of biocatalysts for design to production must be created from biological diverse sources and through protein engineering. Robust and efficient multi-step enzymatic reaction cascades must be developed that can operate simultaneously in one-pot. For this to happen, bio-orthogonal strategies for spatial and temporal control of biocatalyst activities must be developed. Promising approaches and technologies are emerging that will eventually lead to the design of in vitro biocatalytic systems that mimic the metabolic pathways and networks of cellular systems which will be discussed in this roadmap.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.