Evidence map›Paper›PMID 27417730›Full record

ArticleDoklady. Biochemistry and biophysics2016

Synthesis and study on activity in vitro of the high purity human butyrylcholinesterase conjugated with gold nanoparticles.

O I Sokolov, N Y Selivanov, V A Bogatyrev, O G Selivanova, Y I Velikorodnaya, A Y Pocheptsov, B N Filatov, S Y Shchyogolev, L A Dykman

Abstract read
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In one paragraph

Article in Doklady. Biochemistry and biophysics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

O I SokolovInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia. sokolov@ibppm.sgu.ru.
N Y SelivanovInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
V A BogatyrevInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
O G SelivanovaInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
Y I VelikorodnayaResearch Institute of Hygiene, Toxicology, and Occupational Pathology Federal Biomedical Agency, ul. Zemlyachki 12, Volgograd, 400048, Russia.
A Y PocheptsovResearch Institute of Hygiene, Toxicology, and Occupational Pathology Federal Biomedical Agency, ul. Zemlyachki 12, Volgograd, 400048, Russia.
B N FilatovResearch Institute of Hygiene, Toxicology, and Occupational Pathology Federal Biomedical Agency, ul. Zemlyachki 12, Volgograd, 400048, Russia.
S Y ShchyogolevInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
L A DykmanInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
Institute of Biochemistry and Physiology of Plants and Microorganisms · RUFederal Medical-Biological Agency · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plasma was modified. The synthesis of 15-nm gold nanoparticles was carried out by citrated method. A method of conjugation of the high-purity butyrylcholinesterase with gold nanoparticles was developed. It was found that the Immobilization of butyrylcholinesterase on the surface of gold nanoparticles resulted in a significant (to 23%) increase in the specific activity of the enzyme.

Indexed as

ButyrylcholinesteraseMetal NanoparticlesEnzyme StabilityGold CompoundsHumansHydrogen-Ion ConcentrationParticle SizeButyrylcholinesteraseGold Compounds

Identifiers

PMID27417730
OpenAlexW2470402275

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.