ArticleScientific reports2016
Dynamics of plant DNA replication based on PCNA visualization.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Molecular regulation of plant endoreduplication: core mechanisms and their upstream control.Plant molecular biology · 2026Review
- Cell cycle follows 'pause and play' mechanism in salt and cold stress recovery in diverse plant species.The New phytologist · 2026Article
- Molecular markers in cell cycle visualisation during development and stress conditions inQuantitative plant biology · 2024Article
- Cell cycle status of male and female gametes during Arabidopsis reproduction.Plant physiology · 2023Article
- A mutation in THREONINE SYNTHASE 1 uncouples proliferation and transition domains of the root apical meristem: experimental evidence and in silico proposed mechanism.Development (Cambridge, England) · 2022Article
- A hierarchical transcriptional network activates specific CDK inhibitors that regulate G2 to control cell size and number in Arabidopsis.Nature communications · 2022Article
- Zebularine induces enzymatic DNA-protein crosslinks in 45S rDNA heterochromatin of Arabidopsis nuclei.Nucleic acids research · 2022Article
- Improved clearing method contributes to deep imaging of plant organs.Communications biology · 2022Article
- Tools for Assessing Cell-Cycle Progression in Plants.Plant & cell physiology · 2021Review
- A perspective on cell proliferation kinetics in the root apical meristem.Journal of experimental botany · 2021Review
- A novel evaluation method for Ki-67 immunostaining in paraffin-embedded tissues.Virchows Archiv : an international journal of pathology · 2021Article
- Article
- Dynamic Stereoselection of Peptide Helicates and Their Selective Labeling of DNA Replication Foci in Cells*.Angewandte Chemie (International ed. in English) · 2021Article
- The Armadillo BTB Protein ABAP1 Is a Crucial Player in DNA Replication and Transcription of Nematode-Induced Galls.Frontiers in plant science · 2021Article
- Understanding 3D Genome Organization and Its Effect on Transcriptional Gene Regulation Under Environmental Stress in Plant: A Chromatin Perspective.Frontiers in cell and developmental biology · 2021Review
- Caught in the Act: Live-Cell Imaging of Plant Meiosis.Frontiers in plant science · 2021Review
- 5-Aminouracil and other inhibitors of DNA replication induce biphasic interphase-mitotic cells in apical root meristems of Allium cepa.Plant cell reports · 2020Article
- Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy.eLife · 2020Article
- Heat and chilling stress induce nucleolus morphological changes.Journal of plant research · 2019Article
- Archaeal imaging: leading the hunt for new discoveries.Molecular biology of the cell · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA replication is an essential process for the copying of genomic information in living organisms. Imaging of DNA replication in tissues and organs is mainly performed using fixed cells after incorporation of thymidine analogs. To establish a useful marker line to measure the duration of DNA replication and analyze the dynamics of DNA replication, we focused on the proliferating cell nuclear antigen (PCNA), which functions as a DNA sliding clamp for replicative DNA polymerases and is an essential component of replisomes. In this study we produced an Arabidopsis thaliana line expressing PCNA1 fused with the green fluorescent protein under the control of its own promoter (pAtPCNA1::AtPCNA1-sGFP). The duration of the S phase measured using the expression line was consistent with that measured after incorporation of a thymidine analog. Live cell imaging revealed that three distinct nuclear localization patterns (whole, dotted, and speckled) were sequentially observable. These whole, dotted, and speckled patterns of subnuclear AtPCNA1 signals were indicative of the G1 or G2 phase, early S phase and late S phase, respectively. The results indicate that the pAtPCNA1::AtPCNA1-sGFP line is a useful marker line for visualization of S-phase progression in live plant organs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.