ArticlePLoS pathogens2016
Biosynthesis of Antibiotic Leucinostatins in Bio-control Fungus Purpureocillium lilacinum and Their Inhibition on Phytophthora Revealed by Genome Mining.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed.
- Purpureocillium lilacinum and Pochonia chlamydosporia as biocontrol agents: a comparative review of efficacy, ecology, and integrated application.Archives of microbiology · 2026Review
- Comparative Genomics Analysis Reveals the Genomic Basis of S8 Proteases, CAZymes, and Secondary Metabolism Associated with Nematode Biocontrol inInternational journal of molecular sciences · 2026Article
- Whole Genome Sequencing, Comparative Genome Analysis, and Biotechnological Potential ofJournal of fungi (Basel, Switzerland) · 2026Article
- Genomic analyses of Purpureocillium lilacinum reveal unique DNA regions for developing isolate-specific molecular markers.BMC genomics · 2025Article
- Article
- Biosynthesis of the Fungal Cyclic Lipodepsipeptide Pleosporacin, a New Selective Inhibitor of the Phytopathogen Botrytis cinerea.Chembiochem : a European journal of chemical biology · 2025Article
- Metarhizium spp. encode an ochratoxin cluster and a high efficiency ochratoxin-degrading amidohydrolase revealed by genomic analysis.Journal of advanced research · 2025Article
- Dissecting Structural Requirements of Leucinostatin A Derivatives for Antiprotozoal Activity and Mammalian Toxicity.Journal of medicinal chemistry · 2025Article
- Unveiling the Genomic Features and Biocontrol Potential ofJournal of fungi (Basel, Switzerland) · 2025Article
- Leucinostatins target Plasmodium mitochondria to block malaria transmission.Parasites & vectors · 2024Article
- The neglected giants: Uncovering the prevalence and functional groups of huge proteins in proteomes.PLoS computational biology · 2024Article
- Influence of Physicochemical Properties of Iron Oxide Nanoparticles on Their Antibacterial Activity.ACS omega · 2024Review
- The Catalase GeneJournal of fungi (Basel, Switzerland) · 2024Article
- Characterization of a methyltransferase for iterative N-methylation at the leucinostatin termini in Purpureocillium lilacinum.Communications biology · 2024Article
- Article
- Identification of immunostimulatory activities and active compounds from sequentially extracted fractions of rhizosphere fungal fermentation broth ofFrontiers in pharmacology · 2024Article
- Insights into the efficient degradation mechanism of extracellular proteases mediated byFrontiers in microbiology · 2024Article
- The sensor histidine kinase (SLN1) and acetyl-CoA carboxylase (ACC1) coordinately regulate the response ofApplied and environmental microbiology · 2023Article
- Applications and Market of Micro-Organism-Based and Plant-Based Inputs in Brazilian Agriculture.Plants (Basel, Switzerland) · 2023Review
- Poly(butylene adipate-co-terephthalate) biodegradation by Purpureocillium lilacinum strain BA1S.Applied microbiology and biotechnology · 2023Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpureocillium lilacinum of Ophiocordycipitaceae is one of the most promising and commercialized agents for controlling plant parasitic nematodes, as well as other insects and plant pathogens. However, how the fungus functions at the molecular level remains unknown. Here, we sequenced two isolates (PLBJ-1 and PLFJ-1) of P. lilacinum from different places Beijing and Fujian. Genomic analysis showed high synteny of the two isolates, and the phylogenetic analysis indicated they were most related to the insect pathogen Tolypocladium inflatum. A comparison with other species revealed that this fungus was enriched in carbohydrate-active enzymes (CAZymes), proteases and pathogenesis related genes. Whole genome search revealed a rich repertoire of secondary metabolites (SMs) encoding genes. The non-ribosomal peptide synthetase LcsA, which is comprised of ten C-A-PCP modules, was identified as the core biosynthetic gene of lipopeptide leucinostatins, which was specific to P. lilacinum and T. ophioglossoides, as confirmed by phylogenetic analysis. Furthermore, gene expression level was analyzed when PLBJ-1 was grown in leucinostatin-inducing and non-inducing medium, and 20 genes involved in the biosynthesis of leucionostatins were identified. Disruption mutants allowed us to propose a putative biosynthetic pathway of leucinostatin A. Moreover, overexpression of the transcription factor lcsF increased the production (1.5-fold) of leucinostatins A and B compared to wild type. Bioassays explored a new bioactivity of leucinostatins and P. lilacinum: inhibiting the growth of Phytophthora infestans and P. capsici. These results contribute to our understanding of the biosynthetic mechanism of leucinostatins and may allow us to utilize P. lilacinum better as bio-control agent.
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