ArticlePLoS neglected tropical diseases2016
Strong-LAMP: A LAMP Assay for Strongyloides spp. Detection in Stool and Urine Samples. Towards the Diagnosis of Human Strongyloidiasis Starting from a Rodent Model.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 36 citations in OpenAlex.
- Prevalence of strongyloidiasis in the general population of the world: a systematic review and meta-analysis.Pathogens and global health · 2021Pooled it
- An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how?Parasitology · 2020Pooled it
- Development of loop mediated isothermal amplification assay for rapid detection and its comparison with PCR techniques for Ancylostoma duodenale.Scientific reports · 2025Article
- The Recombinase Polymerase Amplification Test forPathogens (Basel, Switzerland) · 2024Article
- First field study using Strong-LAMP for diagnosis of strongyloidiasis in Cubal, Angola.Parasites & vectors · 2023Article
- Development of a Duplex LAMP Assay with Probe-Based Readout for Simultaneous Real-Time Detection ofInternational journal of molecular sciences · 2023Article
- Article
- LAMP Assay for the Detection ofPathogens (Basel, Switzerland) · 2021Article
- LAMP in Neglected Tropical Diseases: A Focus on Parasites.Diagnostics (Basel, Switzerland) · 2021Review
- Direct detection of Strongyloides infection via molecular and antigen detection methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Review
- Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections.International journal of molecular sciences · 2020Review
- Article
- Comparison of Loop-Mediated Isothermal Amplification and Real-Time PCR Assays for Detection of Strongyloides Larvae in Different Specimen Matrices.Journal of clinical microbiology · 2019Article
- Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook.Infectious diseases of poverty · 2019Review
- Strongyloidiasis: A Neglected Tropical Disease.Infectious disease clinics of North America · 2019Review
- Article
- Strongyloides stercoralis: detection of parasite-derived DNA in serum samples obtained from immunosuppressed patients.Parasitology research · 2018Article
- A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples.PLoS neglected tropical diseases · 2018Article
- Characterization of the complete mitochondrial genome of Marshallagia marshalli and phylogenetic implications for the superfamily Trichostrongyloidea.Parasitology research · 2018Article
- Isothermal diagnostic assays for the detection of soil-transmitted helminths based on the SmartAmp2 method.Parasites & vectors · 2017Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStrongyloides stercoralis, the chief causative agent of human strongyloidiasis, is a nematode globally distributed but mainly endemic in tropical and subtropical regions. Chronic infection is often clinically asymptomatic but it can result in severe hyperinfection syndrome or disseminated strongyloidiasis in immunocompromised patients. There is a great diversity of techniques used in diagnosing the disease, but definitive diagnosis is accomplished by parasitological examination of stool samples for morphological identification of parasite. Until now, no molecular method has been tested in urine samples as an alternative to stool samples for diagnosing strongyloidiasis. This study aimed to evaluate the use of a new molecular LAMP assay in a well-established Wistar rat experimental infection model using both stool and, for the first time, urine samples. The LAMP assay was also clinically evaluated in patients´ stool samples. METHODOLOGY/PRINCIPAL
findingsStool and urine samples were obtained daily during a 28-day period from rats infected subcutaneously with different infective third-stage larvae doses of S. venezuelensis. The dynamics of parasite infection was determined by daily counting the number of eggs per gram of feces from day 1 to 28 post-infection. A set of primers for LAMP assay based on a DNA partial sequence in the 18S rRNA gene from S. venezuelensis was designed. The set up LAMP assay (namely, Strong-LAMP) allowed the sensitive detection of S. venezuelensis DNA in both stool and urine samples obtained from each infection group of rats and was also effective in S. stercoralis DNA amplification in patients´ stool samples with previously confirmed strongyloidiasis by parasitological and real-time PCR tests. CONCLUSIONS/SIGNIFICANCE: Our Strong-LAMP assay is an useful molecular tool in research of a strongyloidiasis experimental infection model in both stool and urine samples. After further validation, the Strong-LAMP could also be potentially applied for effective diagnosis of strongyloidiasis in a clinical setting.
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