ArticlePloS one2016
Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 226 citations in OpenAlex.
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- In Vitro Assessment of Essential Oils for Their Methane Mitigation Potential and Impact on Rumen Fermentation in Cattle.Animals : an open access journal from MDPI · 2026Article
- Atomic force microscopy reveals antibacterial mechanisms of Hermetia illucens fatty acids against MDR bacteria.Applied microbiology and biotechnology · 2025Article
- Toxicological assessment of citral and geraniol: Efflux pump inhibition inToxicology reports · 2025Article
- Comprehensive investigation ofNarra J · 2025Article
- Magnolia essential oil: a preliminary exploration of chemical composition and its antimicrobial and antioxidant potential.Frontiers in microbiology · 2025Article
- Monoterpenoid synergy: a new frontier in biological applications.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- Molecular Docking and anti-MRSA effects of bioactive compounds fromIn silico pharmacology · 2025Article
- Efficacy of an Edible Coating with Carvacrol and Citral in Frozen Strawberries and Blueberries to Control Foodborne Pathogens.Foods (Basel, Switzerland) · 2024Article
- Methods for Determination of Antimicrobial Activity of Essential Oils In Vitro-A Review.Plants (Basel, Switzerland) · 2024Review
- Article
- Synergistic anti-virulence efficacy of citral and carvacrol against mixed vaginitis causing Candida albicans and Gardnerella vaginalis: An in vitro and in vivo study.The Journal of antibiotics · 2024Article
- Expanding Knowledge about the Influence of Citral on Cognitive Functions-In Vitro, In Vivo and Ex Vivo Studies.International journal of molecular sciences · 2024Article
- Pathological and pharmacovigilance monitoring as toxicological imputations of azithromycin and its residues in broilers.Veterinary world · 2024Article
- Screening and In Silico Analyses of the YeastFoods (Basel, Switzerland) · 2024Article
- Untargeted metabolomics uncovers prime pathways linked to antibacterial action of citral against bacterial vaginosis-causingHeliyon · 2024Article
- Disclosing the potential of Cupressus leylandii A.B. Jacks & Dallim, Eucalyptus globulus Labill., Aloysia citrodora Paláu, and Melissa officinalis L. hydrosols as eco-friendly antimicrobial agents.Natural products and bioprospecting · 2024Article
- Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Citral is a flavor component that is commonly used in food, beverage and fragrance industries. Cronobacter sakazakii is a food-borne pathogen associated with severe illness and high mortality in neonates and infants. The objective of the present study was to evaluate antimicrobial effect of citral against C. sakazakii strains. The minimum inhibitory concentration (MIC) of citral against C. sakazakii was determined via agar dilution method, then Gompertz models were used to quantitate the effect of citral on microbial growth kinetics. Changes in intracellular pH (pHin), membrane potential, intracellular ATP concentration, and membrane integrity were measured to elucidate the possible antimicrobial mechanism. Cell morphology changes were also examined using a field emission scanning electron microscope. The MICs of citral against C. sakazakii strains ranged from 0.27 to 0.54 mg/mL, and citral resulted in a longer lag phase and lower growth rate of C. sakazakii compared to the control. Citral affected the cell membrane of C. sakazakii, as evidenced by decreased intracellular ATP concentration, reduced pHin, and cell membrane hyperpolarization. Scanning electron microscopy analysis further confirmed that C. sakazakii cell membranes were damaged by citral. These findings suggest that citral exhibits antimicrobial effect against C. sakazakii strains and could be potentially used to control C. sakazakii in foods. However, how it works in food systems where many other components may interfere with its efficacy should be tested in future research before its real application.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.