ArticlePloS one2016
Entamoeba histolytica Trophozoites and Lipopeptidophosphoglycan Trigger Human Neutrophil Extracellular Traps.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Unraveling the interplay between unicellular parasites and bacterial biofilms: Implications for disease persistence and antibiotic resistance.Virulence · 2024Review
- The CAMKK/AMPK Pathway Contributes toInternational journal of molecular sciences · 2024Article
- Neutrophils versus Protozoan Parasites:Microorganisms · 2024Review
- Revisiting the isolation and characterisation of Entamoeba histolytica lipopeptidophosphoglycan.Parasitology research · 2024Article
- Article
- Pathogenicity and virulence ofVirulence · 2023Review
- NETosis in Parasitic Infections: A Puzzle That Remains Unsolved.International journal of molecular sciences · 2023Review
- Diversity and Plasticity of Virulent Characteristics ofTropical medicine and infectious disease · 2023Review
- Review
- Trichomonas vaginalis triggers neutrophil extracellular traps reducing parasite integrity and growth.Parasitology research · 2022Article
- Significance of Mast Cell Formed Extracellular Traps in Microbial Defense.Clinical reviews in allergy & immunology · 2022Review
- Immunomodulatory effect of extracellular vesicles fromFrontiers in cellular and infection microbiology · 2022Article
- Gasdermins in Innate Host Defense AgainstFrontiers in immunology · 2022Review
- Molecular interplays of theFrontiers in cellular and infection microbiology · 2022Review
- New Insights on NETosis Induced byAntioxidants (Basel, Switzerland) · 2021Article
- Host Protective Mechanisms to Intestinal Amebiasis.Trends in parasitology · 2021Review
- The state of art of neutrophil extracellular traps in protozoan and helminthic infections.Bioscience reports · 2019Review
- Non-vesicular Lipid Transport Machinery inFrontiers in cellular and infection microbiology · 2018Review
- Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neutrophil defense mechanisms include phagocytosis, degranulation and the formation of extracellular traps (NET). These networks of DNA are triggered by several immune and microbial factors, representing a defense strategy to prevent microbial spread by trapping/killing pathogens. This may be important against Entamoeba histolytica, since its large size hinders its phagocytosis. The aim of this study was to determine whether E. histolytica and their lipopeptidophosphoglycan (EhLPPG) induce the formation of NETs and the outcome of their interaction with the parasite. Our data show that live amoebae and EhLPPG, but not fixed trophozoites, induced NET formation in a time and dose dependent manner, starting at 5 min of co-incubation. Although immunofluorescence studies showed that the NETs contain cathelicidin LL-37 in close proximity to amoebae, the trophozoite growth was only affected when ethylene glycol tetra-acetic acid (EGTA) was present during contact with NETs, suggesting that the activity of enzymes requiring calcium, such as DNases, may be important for amoeba survival. In conclusion, E. histolytica trophozoites and EhLPPG induce in vitro formation of human NETs, which did not affect the parasite growth unless a chelating agent was present. These results suggest that NETs may be an important factor of the innate immune response during infection with E. histolytica.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.