ArticleProceedings of the National Academy of Sciences of the United States of America2016
A conserved amino acid residue critical for product and substrate specificity in plant triterpene synthases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
- Genome-Wide Identification and Expression Profiling of the Oxidosqualene Cyclase Gene Family inCurrent issues in molecular biology · 2026Article
- Mechanistic Insights Into Functional Innovations of Dammarenediol-II Synthase in Panax ginseng.Plant biotechnology journal · 2026Article
- Ginsenosides in Modern Pharmaceutics: Mechanisms, Applications, Challenges, and Perspectives.Biomolecules · 2026Review
- Functional characterization of 2,3-oxidosqualene cyclase8 in Taraxacum mongolicum: overexpression enhances taraxasterol biosynthesis and antioxidant capacity.BMC genomics · 2026Article
- Identification of 2,3-oxidosqualene cyclase gene inHorticulture research · 2025Article
- Expansion of the Stereochemical Space of Triterpenes by Mining Noncanonical Oxidosqualene Cyclases Across the Diversity of Green Plants.Journal of the American Chemical Society · 2025Article
- Comprehensive mutant chemotyping reveals embedding of a lineage-specific biosynthetic gene cluster in wider plant metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Class II terpene cyclases: structures, mechanisms, and engineering.Natural product reports · 2024Review
- Seasonal variations of triterpene acid contents in Viscum album L. on typical host trees of Hyrcanian forests.Scientific reports · 2023Article
- Discovering a uniform functional trade-off of the CBC-type 2,3-oxidosqualene cyclases and deciphering its chemical logic.Science advances · 2023Article
- Identification of oxidosqualene cyclases associated with saponin biosynthesis from Astragalus membranaceus reveals a conserved motif important for catalytic function.Journal of advanced research · 2023Article
- Biosynthesis of saponin defensive compounds in sea cucumbers.Nature chemical biology · 2022Article
- A conserved mechanism affecting hydride shifting and deprotonation in the synthesis of hopane triterpenes as compositions of wax in oat.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Reciprocal mutations of two multifunctional β-amyrin synthases from Barbarea vulgaris shift α/β-amyrin ratios.Plant physiology · 2022Article
- Plant triterpenoid scaffolding: A tale of two cyclases.Plant physiology · 2022Article
- The Methionine 549 and Leucine 552 Residues of Friedelin Synthase fromMolecules (Basel, Switzerland) · 2021Article
- Postharvest Drying Techniques Regulate Secondary Metabolites and Anti-Neuroinflammatory Activities ofMolecules (Basel, Switzerland) · 2021Article
- Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits.Horticulture research · 2021Article
- Engineering Production of a Novel Diterpene Synthase Precursor inFrontiers in plant science · 2021Article
- Peptide Side-COOH Groups Have Two Distinct Conformations under Biorelevant Conditions.The journal of physical chemistry letters · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Triterpenes are structurally complex plant natural products with numerous medicinal applications. They are synthesized through an origami-like process that involves cyclization of the linear 30 carbon precursor 2,3-oxidosqualene into different triterpene scaffolds. Here, through a forward genetic screen in planta, we identify a conserved amino acid residue that determines product specificity in triterpene synthases from diverse plant species. Mutation of this residue results in a major change in triterpene cyclization, with production of tetracyclic rather than pentacyclic products. The mutated enzymes also use the more highly oxygenated substrate dioxidosqualene in preference to 2,3-oxidosqualene when expressed in yeast. Our discoveries provide new insights into triterpene cyclization, revealing hidden functional diversity within triterpene synthases. They further open up opportunities to engineer novel oxygenated triterpene scaffolds by manipulating the precursor supply.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.