ArticleNature communications2016
Structural basis of transcobalamin recognition by human CD320 receptor.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- [Pathophysiology of hypervitaminosis B12: from origin to clinical event. Scoping review].Revista medica del Instituto Mexicano del Seguro Social · 2026Article
- Inherited disorders of cobalamin metabolism in childhood: biochemical and clinical perspectives.Frontiers in nutrition · 2026Review
- Vitamin B12 and Affective Disorders: A Focus on the Gut-Brain Axis.Alpha psychiatry · 2025Review
- CD320 Receptor and Vitamin B12 as Potential Targets for Anti-Cancer Therapy.International journal of molecular sciences · 2025Review
- Vitamin B12 Levels Association with Functional and Structural Biomarkers of Central Nervous System Injury in Older Adults.Annals of neurology · 2025Article
- Serum Transcobalamin Concentration in Cats-Method Validation and Evaluation in Chronic Enteropathies and Other Conditions.Veterinary sciences · 2024Article
- Vitamin BInternational journal of molecular sciences · 2024Review
- Gene-vegetarianism interactions in calcium, estimated glomerular filtration rate, and testosterone identified in genome-wide analysis across 30 biomarkers.PLoS genetics · 2024Article
- Transcobalamin receptor antibodies in autoimmune vitamin B12 central deficiency.Science translational medicine · 2024Article
- Functional regulation of microglia by vitamin B12 alleviates ischemic stroke-induced neuroinflammation in mice.iScience · 2024Article
- FTY720 requires vitamin BCell reports · 2023Article
- Article
- Defining the Cell Surface Cysteinome using Two-step Enrichment Proteomics.bioRxiv : the preprint server for biology · 2023Article
- Vitamin B12 Status in Recreational Users of Nitrous Oxide: A Systematic Review Focusing on the Prevalence of Laboratory Abnormalities.Antioxidants (Basel, Switzerland) · 2023Review
- Article
- Bioprocess Strategies for Vitamin BBioengineering (Basel, Switzerland) · 2022Review
- A Historical Review of Brain Drug Delivery.Pharmaceutics · 2022Review
- Probing the functional consequence and clinical relevance of CD320 p.E88del, a variant in the transcobalamin receptor gene.American journal of medical genetics. Part A · 2022Article
- Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Response of Human Glioblastoma Cells to Vitamin B12 Deficiency: A Study Using the Non-Toxic Cobalamin Antagonist.Biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular uptake of vitamin B12 (cobalamin) requires capture of transcobalamin (TC) from the plasma by CD320, a ubiquitous cell surface receptor of the LDLR family. Here we present the crystal structure of human holo-TC in complex with the extracellular domain of CD320, visualizing the structural basis of the TC-CD320 interaction. The observed interaction chemistry can rationalize the high affinity of CD320 for TC and lack of haptocorrin binding. The in vitro affinity and complex stability of TC-CD320 were quantitated using a solid-phase binding assay and thermostability analysis. Stable complexes with TC were also observed for the disease-causing CD320ΔE88 mutant and for the isolated LDLR-A2 domain. We also determined the structure of the TC-CD320ΔE88 complex, which revealed only minor changes compared with the wild-type complex. Finally, we demonstrate significantly reduced in vitro affinity of TC for CD320 at low pH, recapitulating the proposed ligand release during the endocytic pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.