ArticleScientific reports2016
Selective local lysis and sampling of live cells for nucleic acid analysis using a microfluidic probe.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 53 citations in OpenAlex.
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- Pixelated Microfluidics for Drug Screening on Tumour Spheroids and Ex Vivo Microdissected Tumour Explants.Cancers · 2023Article
- Microenvironmental Analysis and Control for Local Cells under Confluent Conditions via a Capillary-Based Microfluidic Device.Analytical chemistry · 2022Article
- Spatially resolved transcriptomics provide a new method for cancer research.Journal of experimental & clinical cancer research : CR · 2022Review
- Nanoneedle-Based Materials for Intracellular Studies.Advances in experimental medicine and biology · 2021Article
- Spatial protein heterogeneity analysis in frozen tissues to evaluate tumor heterogeneity.PloS one · 2021Article
- Biphasic Liquid Microjunction Extraction for Profiling Neuronal RNA Modifications by Liquid Chromatography-Tandem Mass Spectrometry.Analytical chemistry · 2020Article
- A Microfluidic Probe Integrated Device for Spatiotemporal 3D Chemical Stimulation in Cells.Micromachines · 2020Article
- Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity.Nucleic acids research · 2020Article
- Rapid micro-immunohistochemistry.Microsystems & nanoengineering · 2020Article
- User-defined local stimulation of live tissue through a movable microfluidic port.Lab on a chip · 2018Article
- Hydrodynamics in Cell Studies.Chemical reviews · 2018Review
- Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.Micromachines · 2017Review
- Recent Advances in the Analysis of Single Cells.Analytical chemistry · 2017Review
- Tissue lithography: Microscale dewaxing to enable retrospective studies on formalin-fixed paraffin-embedded (FFPE) tissue sections.PloS one · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterogeneity is inherent to biology, thus it is imperative to realize methods capable of obtaining spatially-resolved genomic and transcriptomic profiles of heterogeneous biological samples. Here, we present a new method for local lysis of live adherent cells for nucleic acid analyses. This method addresses bottlenecks in current approaches, such as dilution of analytes, one-sample-one-test, and incompatibility to adherent cells. We make use of a scanning probe technology - a microfluidic probe - and implement hierarchical hydrodynamic flow confinement (hHFC) to localize multiple biochemicals on a biological substrate in a non-contact, non-destructive manner. hHFC enables rapid recovery of nucleic acids by coupling cell lysis and lysate collection. We locally lysed ~300 cells with chemical systems adapted for DNA or RNA and obtained lysates of ~70 cells/μL for DNA analysis and ~15 cells/μL for mRNA analysis. The lysates were introduced into PCR-based workflows for genomic and transcriptomic analysis. This strategy further enabled selective local lysis of subpopulations in a co-culture of MCF7 and MDA-MB-231 cells, validated by characteristic E-cadherin gene expression in individually extracted cell types. The developed strategy can be applied to study cell-cell, cell-matrix interactions locally, with implications in understanding growth, progression and drug response of a tumor.
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