ArticleScientific reports2016
RNAi-mediated knockdown of the voltage gated sodium ion channel TcNav causes mortality in Tribolium castaneum.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Exploiting TRP channel diversity in insects: a pathway to next-generation pest management.Archives of toxicology · 2025Review
- Superior target genes and pathways for RNAi-mediated pest control revealed by genome-wide analysis in the beetle Tribolium castaneum.Pest management science · 2025Article
- Article
- The role of polyplexes in developing a green sustainable approach in agriculture.RSC advances · 2022Review
- Guiding RNAi Design Through Characterization of Endogenous Small RNA Pathways.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide.Frontiers in zoology · 2021Review
- Highly Variable Dietary RNAi Sensitivity Among Coleoptera.Frontiers in plant science · 2021Review
- Targeting the potassium ion channel genes SK and SH as a novel approach for control of insect pests: efficacy and biosafety.Pest management science · 2019Article
- RNA interference-mediated knockdown of voltage-gated sodium channel (MpNaScientific reports · 2019Article
- Gene expression inPeerJ · 2019Article
- Complexin in ivermectin resistance in body lice.PLoS genetics · 2018Article
- Guanidinium-Functionalized Interpolyelectrolyte Complexes Enabling RNAi in Resistant Insect Pests.Biomacromolecules · 2018Article
- A Systematic Study of RNAi Effects and dsRNA Stability inInternational journal of molecular sciences · 2018Article
- Transcriptome analysis and identification of P450 genes relevant to imidacloprid detoxification in Bradysia odoriphaga.Scientific reports · 2018Article
- RNA Interference in Insects: Protecting Beneficials and Controlling Pests.Frontiers in physiology · 2018Review
- Bibliometric analysis of recent sodium channel research.Channels (Austin, Tex.) · 2018Article
- Implementing the sterile insect technique with RNA interference - a review.Entomologia experimentalis et applicata · 2017Article
- Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis.PloS one · 2017Article
- RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far.Frontiers in physiology · 2016Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The voltage-gated sodium ion channel (VGSC) belongs to the largest superfamily of ion channels. Since VGSCs play key roles in physiological processes they are major targets for effective insecticides. RNA interference (RNAi) is widely used to analyse gene function, but recently, it has shown potential to contribute to novel strategies for selectively controlling agricultural insect pests. The current study evaluates the delivery of dsRNA targeted to the sodium ion channel paralytic A (TcNav) gene in Tribolium castaneum as a viable means of controlling this insect pest. Delivery of TcNav dsRNA caused severe developmental arrest with larval mortalities up to 73% post injection of dsRNA. Injected larvae showed significant (p < 0.05) knockdown in gene expression between 30-60%. Expression was also significantly (p < 0.05) reduced in pupae following injection causing 30% and 42% knockdown for early and late pupal stages, respectively. Oral delivery of dsRNA caused dose-dependant mortalities of between 19 and 51.34%; this was accompanied by significant (p < 0.05) knockdown in gene expression following 3 days of continuous feeding. The majority of larvae injected with, or fed, dsRNA died during the final larval stage prior to pupation. This work provides evidence of a viable RNAi-based strategy for insect control.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.