ArticlePloS one2016
In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Salinity Stress in Rice: Multilayered Approaches for Sustainable Tolerance.International journal of molecular sciences · 2025Review
- A comprehensive review of in planta stable transformation strategies.Plant methods · 2024Review
- Overexpression of aInternational journal of molecular sciences · 2023Article
- Impact of Nanomaterials on the Regulation of Gene Expression and Metabolomics of Plants under Salt Stress.Plants (Basel, Switzerland) · 2022Review
- Introgression ofCells · 2021Article
- Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos.Journal, genetic engineering & biotechnology · 2021Article
- A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.).Journal, genetic engineering & biotechnology · 2020Article
- Metabolic profiling and scavenging activities of developing circumscissile fruit of psyllium (Plantago ovata Forssk.) reveal variation in primary and secondary metabolites.BMC plant biology · 2020Article
- Review
- Ectopic expression of CPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Review
- The Pyruvate-Phosphate Dikinase (CFrontiers in plant science · 2020Article
- Improved salinity tolerance and growth performance in transgenic sunflower plants via ectopic expression of a wheat antiporter gene (TaNHX2).Molecular biology reports · 2019Article
- Isopentenyl Transferase (IPT) Gene Transfer to Perennial Ryegrass Through Sonication-Assisted Agrobacterium-Mediated Transformation (SAAT), Vacuum and Heat Treatment.Molecular biotechnology · 2019Article
- Article
- Mechanisms of Salt Tolerance in Halophytes: Current Understanding and Recent Advances.Open life sciences · 2018Article
- Co-expression of ArabidopsisFrontiers in plant science · 2017Article
- Halophytes: Potential Resources for Salt Stress Tolerance Genes and Promoters.Frontiers in plant science · 2017Review
- Overexpression of a Plasma Membrane-LocalizedFrontiers in plant science · 2017Article
- A novel transcription factor-like gene SbSDR1 acts as a molecular switch and confers salt and osmotic endurance to transgenic tobacco.Scientific reports · 2016Article
- Overexpression of a Plasma Membrane Bound NaFrontiers in plant science · 2016Article
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4 authors.
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Abstract
Cumin is an annual, herbaceous, medicinal, aromatic, spice glycophyte that contains diverse applications as a food and flavoring additive, and therapeutic agents. An efficient, less time consuming, Agrobacterium-mediated, a tissue culture-independent in planta genetic transformation method was established for the first time using cumin seeds. The SbNHX1 gene, cloned from an extreme halophyte Salicornia brachiata was transformed in cumin using optimized in planta transformation method. The SbNHX1 gene encodes a vacuolar Na+/H+ antiporter and is involved in the compartmentalization of excess Na+ ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII) specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. Transgenic lines L3 and L13 showed high expression of the SbNHX1 gene compared to L6 whereas moderate expression was detected in L5 and L10 transgenic lines. Transgenic lines (L3, L5, L10 and L13), overexpressing the SbNHX1 gene, showed higher photosynthetic pigments (chlorophyll a, b and carotenoid), and lower electrolytic leakage, lipid peroxidation (MDA content) and proline content as compared to wild type plants under salinity stress. Though transgenic lines were also affected by salinity stress but performed better compared to WT plants. The ectopic expression of the SbNHX1 gene confirmed enhanced salinity stress tolerance in cumin as compared to wild type plants under stress condition. The present study is the first report of engineering salt tolerance in cumin, so far and the plant may be utilized for the cultivation in saline areas.
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