ArticleBiophysical journal2016
Nanonet Force Microscopy for Measuring Cell Forces.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed.
- Heart-On-a-Chip with Integrated Ultrasoft Mechanosensors for Continuous Measurement of Cell- and Tissue-Scale Contractile Stresses.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Deep learning reveals how cells pull, buckle, and navigate fibrous environments.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Quantifying cell traction forces at the single-fiber scale in 3D: An approach based on deformable photopolymerized fiber arrays.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Understanding and measuring mechanical signals in the tumor stroma.FEBS open bio · 2025Review
- Mechanical cues guide the formation and patterning of 3D spheroids in fibrous environments.PNAS nexus · 2025Article
- Article
- Dendritic cell force-migration coupling on aligned fiber networks.Biophysical journal · 2024Article
- Actin Filaments Couple the Protrusive Tips to the Nucleus through the I-BAR Domain Protein IRSp53 during the Migration of Cells on 1D Fibers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Sculpting Rupture-Free Nuclear Shapes in Fibrous Environments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Article
- Quantitative biophysical metrics for rapid evaluation of ovarian cancer metastatic potential.Molecular biology of the cell · 2022Article
- Integrating mechanical sensor readouts into organ-on-a-chip platforms.Frontiers in bioengineering and biotechnology · 2022Review
- Revisiting tissue tensegrity: Biomaterial-based approaches to measure forces across length scales.APL bioengineering · 2021Review
- Cell Fragment Formation, Migration, and Force Exertion on Extracellular Mimicking Fiber Nanonets.Advanced biology · 2021Article
- Single Cell Forces after Electroporation.ACS nano · 2021Article
- Force-exerting perpendicular lateral protrusions in fibroblastic cell contraction.Communications biology · 2020Article
- Bioenergetics underlying single-cell migration on aligned nanofiber scaffolds.American journal of physiology. Cell physiology · 2020Article
- Inositol polyphosphate multikinase is a metformin target that regulates cell migration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Crosshatch nanofiber networks of tunable interfiber spacing induce plasticity in cell migration and cytoskeletal response.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The influence of physical forces exerted by or felt by cells on cell shape, migration, and cytoskeleton arrangement is now widely acknowledged and hypothesized to occur due to modulation of cellular inside-out forces in response to changes in the external fibrous environment (outside-in). Our previous work using the non-electrospinning Spinneret-based Tunable Engineered Parameters' suspended fibers has revealed that cells are able to sense and respond to changes in fiber curvature and structural stiffness as evidenced by alterations to focal adhesion cluster lengths. Here, we present the development and application of a suspended nanonet platform for measuring C2C12 mouse myoblast forces attached to fibers of three diameters (250, 400, and 800 nm) representing a wide range of structural stiffness (3-50 nN/μm). The nanonet force microscopy platform measures cell adhesion forces in response to symmetric and asymmetric external perturbation in single and cyclic modes. We find that contractility-based, inside-out forces are evenly distributed at the edges of the cell, and that forces are dependent on fiber structural stiffness. Additionally, external perturbation in symmetric and asymmetric modes biases cell-fiber failure location without affecting the outside-in forces of cell-fiber adhesion. We then extend the platform to measure forces of (1) cell-cell junctions, (2) single cells undergoing cyclic perturbation in the presence of drugs, and (3) cancerous single-cells transitioning from a blebbing to a pseudopodial morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.