ArticleBiophysical journal2016
Mode of Action of Antimicrobial Peptides on E. coli Spheroplasts.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Melittin can permeabilize membranes via large transient pores.Nature communications · 2024Article
- New Strategies to Kill Metabolically-Dormant Cells Directly Bypassing the Need for Active Cellular Processes.Antibiotics (Basel, Switzerland) · 2023Review
- Monomer and Oligomer Transition of Zinc Phthalocyanine Is Key for Photobleaching in Photodynamic Therapy.Molecules (Basel, Switzerland) · 2023Article
- Article
- Regulation of Antimicrobial Peptide Activity via Tuning Deformation Fields by Membrane-Deforming Inclusions.International journal of molecular sciences · 2021Article
- Efficacy of Cathelicidin LL-37 in an MRSA Wound Infection Mouse Model.Antibiotics (Basel, Switzerland) · 2021Article
- Are Bacterial Persisters Dormant Cells Only?Frontiers in microbiology · 2021Review
- Action of antimicrobial peptides and cell-penetrating peptides on membrane potential revealed by the single GUV method.Biophysical reviews · 2020Review
- Using fluorescence microscopy to shed light on the mechanisms of antimicrobial peptides.Future medicinal chemistry · 2019Review
- Membrane potential is vital for rapid permeabilization of plasma membranes and lipid bilayers by the antimicrobial peptide lactoferricin B.The Journal of biological chemistry · 2019Article
- Role of Lipid Composition, Physicochemical Interactions, and Membrane Mechanics in the Molecular Actions of Microbial Cyclic Lipopeptides.The Journal of membrane biology · 2019Review
- Melittin-Induced Permeabilization, Re-sealing, and Re-permeabilization of E. coli Membranes.Biophysical journal · 2018Article
- Article
- Molecular State of the Membrane-Active Antibiotic Daptomycin.Biophysical journal · 2017Article
- Action of Antimicrobial Peptides on Bacterial and Lipid Membranes: A Direct Comparison.Biophysical journal · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We investigated the phenomena of antimicrobial peptides (AMPs) directly attacking the cytoplasmic membranes of Escherichia coli spheroplasts. We developed a procedure for fluorescence recovery after photobleaching to examine dye leakage through bacterial membranes as AMPs in solution bound to the membranes. We found that the AMP binding did not increase the apparent membrane area of a spheroplast, contrary to the response of a lipid-bilayer vesicle, which always showed a membrane area expansion by AMP binding. The permeability through the bacterial membrane increased in a sigmoidal fashion as the AMP binding increased in time, exhibiting a cooperative behavior of AMPs. The analysis of fluorescence recovery after photobleaching showed that the fluxes of dye molecules into and out of the cell were consistent with diffusion of molecules through a number of pores that increased with binding of AMPs and then saturated to a steady level. We discovered a new, to our knowledge, experimental parameter called the flux rate that characterizes the AMP-induced permeability of dye molecules through bacterial membranes. The phenomena observed in bacterial membranes are consistent with the pore-forming activities of AMPs previously observed in lipid bilayers. The experimental value of the flux rate per pore is much smaller than a theoretical value that assumes no friction for the dye molecule's permeation through the pore. We believe that experimental studies of the flux rate will be useful for further analysis of AMPs' permeabilization mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.