ArticlePloS one2016
Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 72 citations in OpenAlex.
- Review
- Tandem-sgRNA Provides an Effective Reverse Genetic Approach for Suppression of Streptomyces Biosynthetic Gene Clusters and Secondary Metabolism.ACS synthetic biology · 2026Article
- Evaluation of vector system in Saccharopolyspora erythraea and construction of new replicative vector.Applied microbiology and biotechnology · 2026Article
- Unraveling rare codon bias in Actinomycetota: lineage-specific and 5' terminal enrichment across 1936 genomes.BMC genomics · 2026Article
- Heterologous Expression and CRISPR/Cas9-Assisted Manipulation of the Hybrid Gene Cluster Specifying the Biosynthesis of Meroterpenoids and Phenazines.ACS synthetic biology · 2026Article
- An Improved Transformation-Associated Recombination Cloning Approach for Direct Capturing of Natural Product Biosynthetic Gene Clusters.Microbial biotechnology · 2024Article
- A stable vector for efficient production of heterologous proteins and secondary metabolites in streptomycetes.Applied microbiology and biotechnology · 2022Article
- Genetically engineered bacterium: Principles, practices, and prospects.Frontiers in microbiology · 2022Review
- Review
- Synthetic biology approaches to actinomycete strain improvement.FEMS microbiology letters · 2021Review
- Cryptic Sulfur Incorporation in Thioangucycline Biosynthesis.Angewandte Chemie (International ed. in English) · 2021Article
- Discovery of Mycothiogranaticins from Streptomyces vietnamensis GIMV4.0001 and the Regulatory Effect of Mycothiol on the Granaticin Biosynthesis.Frontiers in chemistry · 2021Article
- Review
- Recent Advances in Strategies for the Cloning of Natural Product Biosynthetic Gene Clusters.Frontiers in bioengineering and biotechnology · 2021Review
- Heterologous expression of the atypical tetracycline chelocardin reveals the full set of genes required for its biosynthesis.Microbial cell factories · 2020Article
- Highly oxygenated angucycline from Streptomyces sp. KCB15JA014.The Journal of antibiotics · 2020Article
- Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal DerivedMicroorganisms · 2020Article
- Protocols for yTREX/Tn5-based gene cluster expression in Pseudomonas putida.Microbial biotechnology · 2020Article
- Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters.Frontiers in bioengineering and biotechnology · 2020Review
- Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge IsolateMicroorganisms · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transformation-associated recombination (TAR) in yeast is a rapid and inexpensive method for cloning and assembly of large DNA fragments, which relies on natural homologous recombination. Two vectors, based on p15a and F-factor replicons that can be maintained in yeast, E. coli and streptomycetes have been constructed. These vectors have been successfully employed for assembly of the grecocycline biosynthetic gene cluster from Streptomyces sp. Acta 1362. Fragments of the cluster were obtained by PCR and transformed together with the "capture" vector into the yeast cells, yielding a construct carrying the entire gene cluster. The obtained construct was heterologously expressed in S. albus J1074, yielding several grecocycline congeners. Grecocyclines have unique structural moieties such as a dissacharide side chain, an additional amino sugar at the C-5 position and a thiol group. Enzymes from this pathway may be used for the derivatization of known active angucyclines in order to improve their desired biological properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.