ArticleBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica2016
Effects of fluoride on proliferation and mineralization in periodontal ligament cells in vitro.
Article in Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 11 citations in OpenAlex.
- Comparative Evaluation of the Impact of Chloride and Fluoride Based Electrolytes on PDL Cells Before and After Electrochemical Dissolution.Journal of pharmacy & bioallied sciences · 2024Article
- Recent Development of Active Ingredients in Mouthwashes and Toothpastes for Periodontal Diseases.Molecules (Basel, Switzerland) · 2021Review
- Gastrodin alleviates bone damage by modulating protein expression and tissue redox state.FEBS open bio · 2020Article
- Hydrofluoric acid treatment of titanium surfaces enhances the proliferation of human gingival fibroblasts.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF added into osteogenic inductive medium. Immunohistochemistry of cell identification, cell proliferation, alkaline phosphatase (ALP) activity assay, Alizarin red S staining and quantitative real-time-polymerase chain reaction (RT-PCR) were performed. Moderate concentrations of NaF (50-500 μmol/L) had pro-proliferation effects, while 500 μmol/L had the best effects. ALP activity and calcium content were significantly enhanced by 10 μmol/L NaF with osteogenic inductive medium. Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. We conclude that moderate concentrations of NaF can stimulate proliferation and mineralization in periodontal ligament cells. These in vitro findings may provide a novel therapeutic approach for acceleration of periodontal regeneration by addition of suitable concentrations of NaF into the medication for periodontitis treatment, i.e., into periodontal packs and tissue patches.
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