Evidence map›Paper›PMID 27409149›Full record

ArticleAmerican journal of respiratory and critical care medicine2017

MicroRNA Profiling Reveals a Role for MicroRNA-218-5p in the Pathogenesis of Chronic Obstructive Pulmonary Disease.

Griet Conickx, Pieter Mestdagh, Francisco Avila Cobos, Fien M Verhamme, Tania Maes, Bart M Vanaudenaerde, Leen J M Seys, Lies Lahousse, Richard Y Kim, Alan C Hsu and 6 more

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in American journal of respiratory and critical care medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed
6.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 133 citations in OpenAlex.

  1. Article
  2. Review
  3. Participation of miRNA-23a-3p in Pulmonary Tuberculosis Through Macrophages via the JAK-STAT Pathway.International journal of chronic obstructive pulmonary disease · 2026
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  14. Optimizing miRNA transfection for screening in precision cut lung slices.American journal of physiology. Lung cellular and molecular physiology · 2024
    Article
  15. Article
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  17. Review
  18. Article
  19. Article
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23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

Griet Conickx1 Laboratory for Translational Research in Obstructive Pulmonary Diseases, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium.
Pieter Mestdagh2 Center for Medical Genetics, Ghent University, Ghent, Belgium.
Francisco Avila Cobos2 Center for Medical Genetics, Ghent University, Ghent, Belgium.
Fien M Verhamme1 Laboratory for Translational Research in Obstructive Pulmonary Diseases, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium.
Tania Maes1 Laboratory for Translational Research in Obstructive Pulmonary Diseases, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium.
Bart M Vanaudenaerde3 Department of Clinical and Experimental Medicine, Laboratory for Respiratory Diseases, Lung Transplantation Unit, KU Leuven-University of Leuven, Leuven, Belgium.
Leen J M Seys1 Laboratory for Translational Research in Obstructive Pulmonary Diseases, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium.
Lies Lahousse1 Laboratory for Translational Research in Obstructive Pulmonary Diseases, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium.
Richard Y Kim4 Priority Research Centres for Asthma and Respiratory Diseases and Healthy Lungs, Hunter Medical Research Institute and The University of Newcastle, Newcastle, New South Wales, Australia; and.
Alan C Hsu4 Priority Research Centres for Asthma and Respiratory Diseases and Healthy Lungs, Hunter Medical Research Institute and The University of Newcastle, Newcastle, New South Wales, Australia; and.
Peter A Wark4 Priority Research Centres for Asthma and Respiratory Diseases and Healthy Lungs, Hunter Medical Research Institute and The University of Newcastle, Newcastle, New South Wales, Australia; and.
Philip M Hansbro4 Priority Research Centres for Asthma and Respiratory Diseases and Healthy Lungs, Hunter Medical Research Institute and The University of Newcastle, Newcastle, New South Wales, Australia; and.
Guy F Joos1 Laboratory for Translational Research in Obstructive Pulmonary Diseases, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium.
Jo Vandesompele2 Center for Medical Genetics, Ghent University, Ghent, Belgium.
Ken R Bracke1 Laboratory for Translational Research in Obstructive Pulmonary Diseases, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium.ORCID 0000-0001-5906-4605
Guy G Brusselle1 Laboratory for Translational Research in Obstructive Pulmonary Diseases, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium.ORCID 0000-0001-7021-8505
Ghent University Hospital · BEHunter Medical Research Institute · AUJohn Hunter Hospital · AUKU Leuven · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

rationaleAberrant expression of microRNAs (miRNAs) can have a detrimental role in disease pathogenesis.

objectivesTo identify dysregulated miRNAs in lung tissue of patients with chronic obstructive pulmonary disease (COPD).

methodsWe performed miRNA and mRNA profiling using high throughput stem-loop reverse-transcriptase quantitative polymerase chain reaction and mRNA microarray, respectively, on lung tissue of 30 patients (screening cohort) encompassing 8 never-smokers, 10 smokers without airflow limitation, and 12 smokers with COPD. Differential expression of miRNA-218-5p (miR-218-5p) was validated by reverse-transcriptase quantitative polymerase chain reaction in an independent cohort of 71 patients, an in vivo murine model of COPD, and primary human bronchial epithelial cells. Localization of miR-218-5p was assessed by in situ hybridization. In vitro and in vivo perturbation of miR-218-5p combined with RNA sequencing and gene set enrichment analysis was used to elucidate its functional role in COPD pathogenesis. MEASUREMENTS AND MAIN

resultsSeveral miRNAs were differentially expressed among the different patient groups. Interestingly, miR-218-5p was significantly down-regulated in smokers without airflow limitation and in patients with COPD compared with never-smokers. Decreased pulmonary expression of miR-218-5p was validated in an independent validation cohort, in cigarette smoke-exposed mice, and in human bronchial epithelial cells. Importantly, expression of miR-218-5p strongly correlated with airway obstruction. Furthermore, cellular localization of miR-218-5p in human and murine lung revealed highest expression of miR-218-5p in the bronchial airway epithelium. Perturbation experiments with a miR-218-5p mimic or inhibitor demonstrated a protective role of miR-218-5p in cigarette smoke-induced inflammation and COPD.

conclusionsWe highlight a role for miR-218-5p in the pathogenesis of COPD.

Indexed as

AdultAgedAnimalsBronchiCase-Control StudiesFemaleGene Expression ProfilingHumansLungMaleMiceMicroRNAsMiddle AgedOligonucleotide Array Sequence AnalysisPulmonary Disease, Chronic ObstructiveRespiratory MucosaMicroRNAsMIRN218 microRNA, humanchronic obstructive pulmonary diseaselungmicroRNAmicroRNA-218

Identifiers

PMID27409149
OpenAlexW2474216046

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.