Dataset of integrin-linked kinase protein: Protein interactions in cardiomyocytes identified by mass spectrometry.

Alexandra Traister, Mingliang Lu, John G Coles, Jason T Maynes

Open access · goldAbstract read

In one paragraph

Article in Data in brief, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 99% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Alexandra TraisterDivision of Cardiovascular Surgery, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8.

Mingliang LuDivision of Cardiovascular Surgery, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8.

John G ColesDivision of Cardiovascular Surgery, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8.

Jason T MaynesDepartment of Anesthesia and Pain Medicine, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8; Departments of Anesthesia and Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 2J7.

Hospital for Sick Children · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using hearts from mice overexpressing integrin linked kinase (ILK) behind the cardiac specific promoter αMHC, we have performed immunoprecipitation and mass spectrometry to identify novel ILK protein:protein interactions that regulate cardiomyocyte activity and calcium flux. Integrin linked kinase complexes were captured from mouse heart lysates using a commercial antibody, with subsequent liquid chromatography tandem mass spectral analysis. Interacting partners were identified using the MASCOT server, and important interactions verified using reverse immunoprecipitation and mass spectrometry. All ILK interacting proteins were identified in a non-biased manner, and are stored in the ProteomeXchange Consortium via the PRIDE partner repository (reference ID PRIDE: PXD001053). The functional role of identified ILK interactions in cardiomyocyte function and arrhythmia were subsequently confirmed in human iPSC-cardiomyocytes.

Indexed as

Calcium regulationCardiologyMass spectrometryProtein:protein interactions

Identifiers

PMID27408918

DOI10.1016/j.dib.2016.03.056

PMCPMC4927968

OpenAlexW2308583965

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.