Translation of Methodology Used In Human Myocardial Imaging to a Sheep Model of Acute Myocardial Infarction.

Elizabeth A Bailey, Dale L Bailey, Stephen Hunyor, Leigh Ladd, George J Bautovich

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Article in Asia Oceania journal of nuclear medicine & biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Elizabeth A BaileyDepartment of Nuclear Medicine, Royal North Shore Hospital, Australia; Discipline of Medical Radiation Sciences, University of Sydney, Australia.

Dale L BaileyDepartment of Nuclear Medicine, Royal North Shore Hospital, Australia; Discipline of Medical Radiation Sciences, University of Sydney, Australia; Sydney Medical School, University of Sydney, Australia.

Stephen HunyorDepartment of Cardiology, Royal North Shore Hospital, Australia; Sydney Medical School, University of Sydney, Australia; Cardiac Technology Centre, North Shore Heart Research Group, Kolling Institute, Australia.

Leigh LaddCardiac Technology Centre, North Shore Heart Research Group, Kolling Institute, Australia; Charles Sturt University, Wagga Wagga, Australia.

George J BautovichDepartment of Nuclear Medicine, Royal North Shore Hospital, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionPre-clinical investigation of stem cells for repairing damaged myocardium predominantly uses rodents, however large animals have cardiac circulation closely resembling the human heart. The aim of this study was to evaluate whether SPECT/CT myocardial perfusion imaging (MPI) could be used for assessing sheep myocardium following an acute myocardial infarction (MI) and response to intervention.

methodsEighteen sheep were enrolled in a pilot study to evaluate [(99m)Tc]-sestamibi MPI at baseline, post-MI and after therapy. Modifications to the standard MPI protocols were developed. All data was reconstructed with OSEM using CT-derived attenuation and scatter correction. Standard analyses were performed and inter-observer agreement was measured using Kappa (κ). Power determined the sample sizes needed to show statistically significant changes due to intervention.

resultsTen sheep completed the full protocol. Data processed was performed with pre-existing hardware and software used in human MPI scanning. No improvement in perfusion was seen in the control group, however improvements of 15%-35% were seen after intra-myocardial stem cell administration. Inter-observer agreement was excellent (К=0.89). Using a target power of 0.9, 28 sheep were required to detect a 10-12% change in perfusion.

conclusionThis study demonstrates the suitability of large animal models for imaging with standard MPI protocols and its feasibility with a manageable number of animals. These protocols could be translated into humans to study the efficacy of stem cell therapy in heart regeneration and repair.

Indexed as

Mesenchymal stem CellsMyocardial perfusion ImagingOvine modelSPECT/CT

Identifiers

PMID27408845

PMCPMC4927046

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