Evidence map›Paper›PMID 27408580›Full record

ArticleZooKeys2016

PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera.

Niklas Wahlberg, Carlos Peña, Milla Ahola, Christopher W Wheat, Jadranka Rota

Abstract read
In one paragraph

Article in ZooKeys, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Niklas WahlbergDepartment of Biology, University of Turku, 20014 Turku, Finland; Department of Biology, Lund University, 223 62 Lund, Sweden.
Carlos PeñaDepartment of Biology, University of Turku, 20014 Turku, Finland.
Milla AholaDepartment of Biology, University of Turku, 20014 Turku, Finland.
Christopher W WheatDepartment of Zoology, Stockholm University, 106 91 Stockholm, Sweden.
Jadranka RotaDepartment of Biology, University of Turku, 20014 Turku, Finland; Department of Biology, Lund University, 223 62 Lund, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.

Indexed as

LepidopteraMolecular systematicsphylogeneticsphylogenomics

Identifiers

PMID27408580
PMCPMC4926658

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.