ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2016
Validation of an assay for quantification of alpha-amylase in saliva of sheep.
Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Preliminary Investigation on Salivary Enzymes of Massese Sheep.Animals : an open access journal from MDPI · 2026Article
- Alpha-Amylase Activity in Feline Saliva: An Analytical Validation of an Automated Assay for Its Measurement and a Pilot Study on Its Changes Following Acute Stress and Due to Urinary Tract Pathologies.Animals : an open access journal from MDPI · 2025Article
- Salivary alpha-amylase: A marker of stress in gynecological residents during a shoulder dystocia simulation scenario.PloS one · 2024Article
- Current methods for stress marker detection in saliva.Journal of pharmaceutical and biomedical analysis · 2020Review
- Where Do We Stand in the Domestic Dog (Frontiers in psychology · 2020Review
- Salivary alpha-amylase as a stress biomarker in diseased dogs.Journal of veterinary science · 2019Article
- An Appraisal of Methods for Measuring Welfare of Grazing Ruminants.Frontiers in veterinary science · 2019Review
- Changes in alpha-amylase activity, concentration and isoforms in pigs after an experimental acute stress model: an exploratory study.BMC veterinary research · 2018Article
- Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation.BMC veterinary research · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to develop a time-resolved immunofluorometric assay (TR-IFMA) for quantification of salivary alpha-amylase in sheep. For that purpose, after the design of the assay, an analytical and a clinical validation were carried out. The analytical validation of the assay showed intra- and inter-assay coefficients of variation (CVs) of 6.1% and 10.57%, respectively and an analytical limit of detection of 0.09 ng/mL. The assay also demonstrated a high level of accuracy, as determined by linearity under dilution. For clinical validation, a model of acute stress testing was conducted to determine whether expected significant changes in alpha-amylase were picked up in the newly developed assay. In that model, 11 sheep were immobilized and confronted with a sheepdog to induce stress. Saliva samples were obtained before stress induction and 15, 30, and 60 min afterwards. Salivary cortisol was measured as a reference of stress level. The results of TR-IFMA showed a significant increase (P < 0.01) in the concentration of alpha-amylase in saliva after stress induction. The assay developed in this study could be used to measure salivary alpha-amylase in the saliva of sheep and this enzyme could be a possible noninvasive biomarker of stress in sheep.
Indexed as
Identifiers
27408332PMC4924553W2498535023What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.