ArticleIndian journal of microbiology2016
Comparative Genomics Reveals Biomarkers to Identify Lactobacillus Species.
Article in Indian journal of microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Evaluation of Human Dental Plaque Lactic Acid Bacilli for Probiotic Potential and Functional Analysis in Relevance to Oral Health.Indian journal of microbiology · 2023Article
- PCR Screening ofIndian journal of microbiology · 2020Article
- Mapping Microbial Capacities for Bioremediation: Genes to Genomics.Indian journal of microbiology · 2020Review
- Bacterial Populations in Subgingival Plaque Under Healthy and Diseased Conditions: Genomic Insights into Oral Adaptation Strategies byIndian journal of microbiology · 2020Article
- Aligning Microbial Biodiversity for Valorization of Biowastes: Conception to Perception.Indian journal of microbiology · 2019Review
- Fish Gut Microbiome: Current Approaches and Future Perspectives.Indian journal of microbiology · 2018Article
- Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease.Indian journal of microbiology · 2017Article
Corrections and comments
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Authors and funding
2 authors.
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Abstract
Bacteria possessing multiple copies of 16S rRNA (rrs) gene demonstrate high intragenomic heterogeneity. It hinders clear distinction at species level and even leads to overestimation of the bacterial diversity. Fifty completely sequenced genomes belonging to 19 species of Lactobacillus species were found to possess 4-9 copies of rrs each. Multiple sequence alignment of 268 rrs genes from all the 19 species could be classified into 20 groups. Lactobacillus sanfranciscensis TMW 1.1304 was the only species where all the 7 copies of rrs were exactly similar and thus formed a distinct group. In order to circumvent the problem of high heterogeneity arising due to multiple copies of rrs, 19 additional genes (732-3645 nucleotides in size) common to Lactobacillus genomes, were selected and digested with 10 Type II restriction endonucleases (RE), under in silico conditions. The following unique gene-RE combinations: recA (1098 nts)-HpyCH4 V, CviAII, BfuCI and RsaI were found to be useful in identifying 29 strains representing 17 species. Digestion patterns of genes-ruvB (1020 nts), dnaA (1368 nts), purA (1290 nts), dnaJ (1140 nts), and gyrB (1944 nts) in combination with REs-AluI, BfuCI, CviAI, Taq1, and Tru9I allowed clear identification of an additional 14 strains belonging to 8 species. Digestion pattern of genes recA, ruvB, dnaA, purA, dnaJ and gyrB can be used as biomarkers for identifying different species of Lactobacillus.
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