ArticleProceedings of the National Academy of Sciences of the United States of America2016
The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 61 citations in OpenAlex.
- Hfq-dependent artificial regulatory small RNAs for targeted gene expression control in the nitrogen-fixingSynthetic and systems biotechnology · 2026Article
- A bacterial ally for nitrogen-fixing biofilm: enhancing the rhizosphere colonization ofApplied and environmental microbiology · 2026Article
- An engineered Pseudomonas stutzeri strain overexpressing the sigma factor AlgU promotes maize growth under salt stress.Applied microbiology and biotechnology · 2026Article
- Distinct Denitrification Phenotypes in Closely Related Bacteria: Clues to Understanding Variations in Nitrite Accumulation Among Stutzerimonas Strains.Environmental microbiology · 2026Article
- Biofertilizer Industry and Research Developments in China: A Mini-Review.Microbial biotechnology · 2025Review
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- Identification and Functional Analysis of ncRNAs Regulating Intrinsic Polymyxin Resistance in FoodborneMicroorganisms · 2024Article
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- Development of modular expression across phylogenetically distinct diazotrophs.Journal of industrial microbiology & biotechnology · 2024Article
- Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.ACS synthetic biology · 2023Article
- What determines symbiotic nitrogen fixation efficiency in rhizobium: recent insights into Rhizobium leguminosarum.Archives of microbiology · 2023Review
- Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity.Microbial cell factories · 2023Article
- Regulation of hierarchical carbon substrate utilization, nitrogen fixation, and root colonization by the Hfq/Crc/CrcZY genes iniScience · 2022Article
- Comparative Genomics of the Genus Pseudomonas Reveals Host- and Environment-Specific Evolution.Microbiology spectrum · 2022Article
- A long noncoding RNA HILinc1 enhances pear thermotolerance by stabilizing PbHILT1 transcripts through complementary base pairing.Communications biology · 2022Article
- Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-FixingMicroorganisms · 2022Article
- Streptomyces can be an excellent plant growth manager.World journal of microbiology & biotechnology · 2022Review
- Post-transcriptional control of bacterial nitrogen metabolism by regulatory noncoding RNAs.World journal of microbiology & biotechnology · 2022Review
- Article
- Delineation of mechanistic approaches of rhizosphere microorganisms facilitated plant health and resilience under challenging conditions.3 Biotech · 2022Review
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Authors and funding
19 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unlike most Pseudomonas, the root-associated bacterium Pseudomonas stutzeri A1501 fixes nitrogen after the horizontal acquisition of a nitrogen-fixing (nif) island. A genome-wide search for small noncoding RNAs (ncRNAs) in P. stutzeri A1501 identified the novel P. stutzeri-specific ncRNA NfiS in the core genome, whose synthesis was significantly induced under nitrogen fixation or sorbitol stress conditions. The expression of NfiS was RNA chaperone Hfq-dependent and activated by the sigma factor RpoN/global nitrogen activator NtrC/nif-specific activator NifA regulatory cascade. The nfiS-deficient mutant displayed reduced nitrogenase activity, as well as increased sensitivity to multiple stresses, such as osmotic and oxidative stresses. Secondary structure prediction and complementation studies confirmed that a stem-loop structure was essential for NfiS to regulate the nitrogenase gene nifK mRNA synthesis and thus nitrogenase activity. Microscale thermophoresis and physiological analysis showed that NfiS directly pairs with nifK mRNA and ultimately enhances nitrogenase activity by increasing the translation efficiency and the half-life of nifK mRNA. Our data also suggest structural and functional divergence of NfiS evolution in diazotrophic and nondiazotrophic backgrounds. It is proposed that NfiS was recruited by nifK mRNA as a novel regulator to integrate the horizontally acquired nif island into host global networks.
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